In vivo evaluation of combined CK2 inhibition and irradiation in human WiDr tumours

• Verfasst von: Zwicker, Felix [VerfasserIn]
• Verfasst von: Hauswald, Henrik [VerfasserIn]
• Verfasst von: Weber, Klaus-Josef [VerfasserIn]
• Verfasst von: Debus, Jürgen [VerfasserIn]
• Verfasst von: Huber, Peter E. [VerfasserIn]
• Titel: In vivo evaluation of combined CK2 inhibition and irradiation in human WiDr tumours
• Verf.angabe: Felix Zwicker, Henrik Hauswald, Klaus-Josef Weber, Jürgen Debus and Peter E. Huber
• E-Jahr: 2021
• Jahr: January 5, 2021
• Umfang: 7 S.
• Fussnoten: Gesehen am 23.02.2021
• Titel Quelle: Enthalten in: In vivo
• Ort Quelle: Kapandriti, Attiki : IIAR, 2004
• Jahr Quelle: 2021
• Band/Heft Quelle: 35(2021), 1, Seite 111-117
• ISSN Quelle: 1791-7549
• DOI: doi:10.21873/invivo.12238
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: CK2 inhibition
• Sach-SW: human WiDr tumours
• Sach-SW: irradiation
• Sach-SW: TBB
• Sach-SW: tetra-bromobenzotriazole
• K10plus-PPN: 1749190028

Abstract

Background/Aim: Casein kinase 2 (CK2) which sustains multiple pro-survival functions in cellular DNA-damage response, is strictly regulated in normal cells but elevated in cancer. CK2 is considered as a potential therapeutic target, and its inhibition has been associated with radiosensitization in mammalian cells in vitro. Here, we investigated potential radiosensitization by CK2 inhibition in vivo. Materials and Methods: The effect of CK2 inhibition in vivo was investigated in human WiDr-xenograft tumours grown subcutaneously on BALB/c nu/nu mice with and without fractionated irradiation. CK2 inhibition was performed using the specific inhibitor tetra-bromobenzotriazole (TBB). Histological examinations included staining for apoptosis and double-strand breaks. Results: Both TBB treatment alone and radiation alone significantly reduced tumour growth, which was reflected by increased apoptosis rates. However, TBB treatment did not boost radiation-induced tumour growth suppression in combined treatment, although the apoptosis rate increased and repair of double-strand breaks was reduced. This was in stark contrast to previous data on in vitro radiosensitization. Conclusion: The absence of radiosensitization by CK2 inhibition should be investigated in different tumour models.