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Status: Bibliographieeintrag

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Verfasst von:Rode, Miriam [VerfasserIn]   i
 Balkow, Sandra [VerfasserIn]   i
 Sobek, Vera [VerfasserIn]   i
 Brehm, Reina [VerfasserIn]   i
 Martin, Praxedis [VerfasserIn]   i
 Kersten, Astrid [VerfasserIn]   i
 Dumrese, Tilman [VerfasserIn]   i
 Stehle, Thomas [VerfasserIn]   i
 Müllbacher, Arno [VerfasserIn]   i
 Wallich, Reinhard [VerfasserIn]   i
 Simon, Markus M. [VerfasserIn]   i
Titel:Perforin and Fas act together in the induction of apoptosis, and both are critical in the clearance of lymphocytic choriomeningitis virus infection
Verf.angabe:Miriam Rode, Sandra Balkow, Vera Sobek, Reina Brehm, Praxedis Martin, Astrid Kersten, Tilman Dumrese, Thomas Stehle, Arno Müllbacher, Reinhard Wallich, and Markus M. Simon
Jahr:2004
Umfang:11 S.
Fussnoten:Gesehen am 24.02.2021
Titel Quelle:Enthalten in: Journal of virology
Ort Quelle:Baltimore, Md. : Soc., 1967
Jahr Quelle:2004
Band/Heft Quelle:78(2004), 22, Seite 12395-12405
ISSN Quelle:1098-5514
Abstract:In this report we questioned the current view that the two principal cytotoxic pathways, the exocytosis and the Fas ligand (FasL)/Fas-mediated pathway, have largely nonoverlapping biological roles. For this purpose we have analyzed the response of mice that lack Fas as well as granzyme A (gzmA) and gzmB (FasxgzmAxB(-/-)) to infection with lymphocytic choriomeningitis virus (LCMV). We show that FasxgzmAxB(-/-) mice, in contrast to B6, Fas(-/-), and gzmAxB(-/-) mice, do not recover from a primary infection with LCMV, in spite of the expression of comparable numbers of LCMV-immune and gamma interferon-producing cytotoxic T lymphocytes (CTL) in all mouse strains tested. Ex vivo-derived FasxgzmAxB(-/-) CTL lacked nucleolytic activity and expressed reduced cytolytic activity compared to B6 and Fas(-/-) CTL. Furthermore, virus-immune CTL with functional FasL and perforin (gzmAxB(-/-)) are more potent in causing target cell apoptosis in vitro than those expressing FasL alone (perfxgzmAxB(-/-)). This synergistic effect of perforin on Fas-mediated nucleolysis of target cells is indicated by the fact that, compared to perfxgzmAxB(-/-) CTL, gzmAxB(-/-) CTL induced (i) an accelerated decrease in mitochondrial transmembrane potential, (ii) increased generation of reactive oxygen species, and (iii) accelerated phosphatidylserine exposure on plasma membranes. We conclude that perforin does not mediate recovery from LCMV by itself but plays a vital role in both gzmA/B and FasL/Fas-mediated CTL activities, including apoptosis and control of viral infections.
DOI:doi:10.1128/JVI.78.22.12395-12405.2004
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext ; Verlag: https://doi.org/10.1128/JVI.78.22.12395-12405.2004
 Volltext: https://jvi.asm.org/content/78/22/12395
 DOI: https://doi.org/10.1128/JVI.78.22.12395-12405.2004
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:Animals
 Antigens, Differentiation
 Apoptosis
 Endopeptidases
 Fas Ligand Protein
 fas Receptor
 Granzymes
 Lymphocytic Choriomeningitis
 Membrane Glycoproteins
 Mice
 Mice, Inbred BALB C
 Perforin
 Pore Forming Cytotoxic Proteins
 Reactive Oxygen Species
 Serine Endopeptidases
 T-Lymphocytes, Cytotoxic
K10plus-PPN:1749301687
Verknüpfungen:→ Zeitschrift

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