In vitro analysis of nucleic acid recognition in B lymphocytes

• Verfasst von: Ziegler, Saskia [VerfasserIn]
• Verfasst von: Bekeredjian-Ding, Isabelle [VerfasserIn]
• Titel: In vitro analysis of nucleic acid recognition in B lymphocytes
• Verf.angabe: Saskia Ziegler, Isabelle Bekeredjian-Ding
• E-Jahr: 2014
• Jahr: 31 May 2014
• Umfang: 10 S.
• Fussnoten: Gesehen am 24.02.2021
• Titel Quelle: Enthalten in: Innate DNA and RNA recognition
• Ort Quelle: New York, NY : Humana Press, 2014
• Jahr Quelle: 2014
• Band/Heft Quelle: (2014), Seite 87-96
• ISBN Quelle: 978-1-4939-0882-0
• DOI: doi:10.1007/978-1-4939-0882-0_9
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: B cells
• Sach-SW: BCR
• Sach-SW: Differentiation
• Sach-SW: Mitogens
• Sach-SW: Proliferation
• Sach-SW: TLR
• K10plus-PPN: 1749304015

Abstract

In contrast to murine B cells, Toll-like receptor (TLR) expression in human B cells is mainly restricted to endosomally localized TLR7 and -9, receptors for RNA and DNA, respectively. Most importantly, B lymphocytes lack classical phagocytic receptors and instead internalize antigen only via the B cell receptor (BCR), a surface immunoglobulin specific for a defined antigen. BCR ligation triggers internalization of particulate antigens and physically associated molecules among them bacterial DNA or RNA. Thereby, this process provides access to endosomal nucleic acid-sensing TLRs. Co-stimulation of BCR and TLR ultimately leads to T cell-independent B cell activation. Here, we explain how this process can be experimentally mimicked in human peripheral blood B cells, e.g., using a microsphere-based system that promotes uptake of nucleic acid-based TLR ligands via BCR engagement.