| |  Online-Ressource |
|---|
| Verfasst von: | Pardo, Julián [VerfasserIn]  |
|---|
| | Bosque, Alberto [VerfasserIn] |
|---|
| | Brehm, Reina [VerfasserIn] |
|---|
| | Wallich, Reinhard [VerfasserIn] |
|---|
| | Naval, Javier [VerfasserIn] |
|---|
| | Müllbacher, Arno [VerfasserIn] |
|---|
| | Anel, Alberto [VerfasserIn] |
|---|
| | Simon, Markus M. [VerfasserIn] |
|---|
| Titel: | Apoptotic pathways are selectively activated by granzyme A and/or granzyme B in CTL-mediated target cell lysis |
|---|
| Verf.angabe: | Julián Pardo, Alberto Bosque, Reina Brehm, Reinhard Wallich, Javier Naval, Arno Müllbacher, Alberto Anel, and Markus M. Simon |
|---|
| Jahr: | 2004 |
|---|
| Umfang: | 12 S. |
|---|
| Fussnoten: | Gesehen am 24.02.2021 |
|---|
| Titel Quelle: | Enthalten in: The journal of cell biology |
|---|
| Ort Quelle: | New York, NY : Rockefeller Univ. Press, 1962 |
|---|
| Jahr Quelle: | 2004 |
|---|
| Band/Heft Quelle: | 167(2004), 3, Seite 457-468 |
|---|
| ISSN Quelle: | 1540-8140 |
|---|
| Abstract: | Purified cytolytic T lymphocyte (CTL) proteases granzyme (gzm)A and gzmB with sublytic dose of perforin (perf) initiate distinct proapoptotic pathways. Their physiological relevance in CTL-mediated target cell apoptosis is elusive. Using ex vivo virus-immune CD8(+) T cells from mice deficient in perf, gzmA and/or gzmB, and the Fas-resistant EL4.F15 tumor target cell, we show that (a) CTL from gzmA(-/-) or gzmB(-/-) mice similarly induced early proapoptotic features, such as phosphatidyl serine (PS) exposure on plasma membrane, Delta Psi(m) loss, and reactive oxygen radical generation, though with distinct kinetics; (b) CTL from gzmA(-/-) but not from gzmB(-/-) mice activate caspase 3 and 9; (c) PS exposure induced by CTL from gzmA(-/-) or gzmB(-/-) mice is prevented, respectively, by caspase inhibitors or by reactive oxygen scavengers without interfering with target cell death; and (d) all gzm-induced apoptotic features analyzed depend critically on perf. Thus, perf is the principal regulator in CTL-mediated and gzm-facilitated intracellular processes. The ability of gzmA and gzmB to induce multiple independent cell death pathways may be the hosts response to circumvent evasion strategies of pathogens and tumors. |
|---|
| DOI: | doi:10.1083/jcb.200406115 |
|---|
| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1083/jcb.200406115 |
|---|
| | Volltext: https://rupress.org/jcb/article/167/3/457/51394 |
|---|
| | DOI: https://doi.org/10.1083/jcb.200406115 |
|---|
| Datenträger: | Online-Ressource |
|---|
| Sprache: | eng |
|---|
| Sach-SW: | Animals |
|---|
| | Apoptosis |
|---|
| | Cell Line, Tumor |
|---|
| | Cells, Cultured |
|---|
| | Cytotoxicity, Immunologic |
|---|
| | Granzymes |
|---|
| | Kinetics |
|---|
| | Membrane Glycoproteins |
|---|
| | Membrane Potentials |
|---|
| | Mice |
|---|
| | Mice, Knockout |
|---|
| | Perforin |
|---|
| | Phosphatidylserines |
|---|
| | Pore Forming Cytotoxic Proteins |
|---|
| | Reactive Oxygen Species |
|---|
| | Serine Endopeptidases |
|---|
| | T-Lymphocytes, Cytotoxic |
|---|
| | Time Factors |
|---|
| K10plus-PPN: | 1749312670 |
|---|
| Verknüpfungen: | → Zeitschrift |
|---|
Apoptotic pathways are selectively activated by granzyme A and/or granzyme B in CTL-mediated target cell lysis / Pardo, Julián [VerfasserIn]; 2004 (Online-Ressource)
