Electron tomography of plasmodium falciparum merozoites reveals core cellular events that underpin erythrocyte invasion

• Verfasst von: Hanssen, Eric [VerfasserIn]
• Verfasst von: Cyrklaff, Marek [VerfasserIn]
• Verfasst von: Frischknecht, Friedrich [VerfasserIn]
• Titel: Electron tomography of plasmodium falciparum merozoites reveals core cellular events that underpin erythrocyte invasion
• Verf.angabe: Eric Hanssen, Chaitali Dekiwadia, David T. Riglar, Melanie Rug, Leandro Lemgruber, Alan F. Cowman, Marek Cyrklaff, Mikhail Kudryashev, Friedrich Frischknecht, Jake Baum and Stuart A. Ralph
• E-Jahr: 2013
• Jahr: 22 March 2013
• Umfang: 16 S.
• Fussnoten: Gesehen am 26.02.2021
• Titel Quelle: Enthalten in: Cellular microbiology
• Ort Quelle: Oxford [u.a.] : Wiley-Blackwell, 1999
• Jahr Quelle: 2013
• Band/Heft Quelle: 15(2013), 9, Seite 1457-1472
• ISSN Quelle: 1462-5822
• DOI: doi:10.1111/cmi.12132
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1749369419

Abstract

Erythrocyte invasion by merozoites forms of the malaria parasite is a key step in the establishment of human malaria disease. To date, efforts to understand cellular events underpinning entry have been limited to insights from non-human parasites, with no studies at sub-micrometer resolution undertaken using the most virulent human malaria parasite, Plasmodium falciparum. This leaves our understanding of the dynamics of merozoite sub-cellular compartments during infectionincomplete, in particular that of the secretory organelles. Using advances in P. falciparum merozoite isolation and new imaging techniques we present a three-dimensional study of invasion using electron microscopy, cryo-electron tomography and cryo-X-ray tomography. We describe the core architectural features of invasion and identify fusion between rhoptries at the commencement of invasion as a hitherto overlooked event that likely provides a critical step that initiates entry. Given the centrality of merozoite organelle proteins to vaccine development, these insights provide a mechanistic framework to understand therapeutic strategies targeted towards the cellular events of invasion.