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| Verfasst von: | Zhang, Yaqing [VerfasserIn]  |
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| | Kuster, David [VerfasserIn] |
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| | Schmidt, Tobias Thomas [VerfasserIn] |
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| | Kirrmaier, Daniel [VerfasserIn] |
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| | Nübel, Gabriele [VerfasserIn] |
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| | Ibberson, David [VerfasserIn] |
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| | Benes, Vladimir [VerfasserIn] |
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| | Hombauer, Hans [VerfasserIn] |
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| | Knop, Michael [VerfasserIn] |
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| | Jäschke, Andres [VerfasserIn] |
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| Titel: | Extensive 5'-surveillance guards against non-canonical NAD-caps of nuclear mRNAs in yeast |
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| Verf.angabe: | Yaqing Zhang, David Kuster, Tobias Schmidt, Daniel Kirrmaier, Gabriele Nübel, David Ibberson, Vladimir Benes, Hans Hombauer, Michael Knop & Andres Jäschke |
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| E-Jahr: | 2020 |
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| Jahr: | 02 November 2020 |
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| Umfang: | 17 S. |
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| Fussnoten: | Gesehen am 09.05.2022 |
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| Titel Quelle: | Enthalten in: Nature Communications |
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| Ort Quelle: | [London] : Nature Publishing Group UK, 2010 |
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| Jahr Quelle: | 2020 |
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| Band/Heft Quelle: | 11(2020), Artikel-ID 5508, Seite 1-17 |
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| ISSN Quelle: | 2041-1723 |
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| Abstract: | The ubiquitous redox coenzyme nicotinamide adenine dinucleotide (NAD) acts as a non-canonical cap structure on prokaryotic and eukaryotic ribonucleic acids. Here we find that in budding yeast, NAD-RNAs are abundant (>1400 species), short (<170 nt), and mostly correspond to mRNA 5′-ends. The modification percentage of transcripts is low (<5%). NAD incorporation occurs mainly during transcription initiation by RNA polymerase II, which uses distinct promoters with a YAAG core motif for this purpose. Most NAD-RNAs are 3′-truncated. At least three decapping enzymes, Rai1, Dxo1, and Npy1, guard against NAD-RNA at different cellular locations, targeting overlapping transcript populations. NAD-mRNAs are not translatable in vitro. Our work indicates that in budding yeast, most of the NAD incorporation into RNA seems to be disadvantageous to the cell, which has evolved a diverse surveillance machinery to prematurely terminate, decap and reject NAD-RNAs. |
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| DOI: | doi:10.1038/s41467-020-19326-3 |
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| URL: | kostenfrei: Volltext ; Verlag: https://doi.org/10.1038/s41467-020-19326-3 |
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| | kostenfrei: Volltext: https://www.nature.com/articles/s41467-020-19326-3 |
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| | DOI: https://doi.org/10.1038/s41467-020-19326-3 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| K10plus-PPN: | 1750232723 |
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| Verknüpfungen: | → Zeitschrift |
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| Lokale URL UB: |  Zum Volltext |
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Extensive 5'-surveillance guards against non-canonical NAD-caps of nuclear mRNAs in yeast / Zhang, Yaqing [VerfasserIn]; 02 November 2020 (Online-Ressource)
