| |  Online-Ressource |
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| Verfasst von: | Gong, Wenjie [VerfasserIn]  |
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| | Wang, Lei [VerfasserIn] |
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| | Stock, Sophia [VerfasserIn] |
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| | Ni, Ming [VerfasserIn] |
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| | Schubert, Maria-Luisa [VerfasserIn] |
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| | Neuber, Brigitte [VerfasserIn] |
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| | Kleist, Christian [VerfasserIn] |
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| | Hückelhoven-Krauss, Angela [VerfasserIn] |
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| | Wu, Depei [VerfasserIn] |
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| | Müller-Tidow, Carsten [VerfasserIn] |
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| | Schmitt, Anita [VerfasserIn] |
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| | Shiku, Hiroshi [VerfasserIn] |
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| | Schmitt, Michael [VerfasserIn] |
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| | Sellner, Leopold [VerfasserIn] |
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| Titel: | Evaluation of production protocols for the generation of NY-ESO-1-specific T cells |
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| Verf.angabe: | Wenjie Gong, Lei Wang, Sophia Stock, Ming Ni, Maria-Luisa Schubert, Brigitte Neuber, Christian Kleist, Angela Hückelhoven-Krauss, Depei Wu, Carsten Müller-Tidow, Anita Schmitt, Hiroshi Shiku, Michael Schmitt and Leopold Sellner |
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| E-Jahr: | 2021 |
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| Jahr: | 14 January 2021 |
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| Fussnoten: | Gesehen am 04.03.2021 |
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| Titel Quelle: | Enthalten in: Cells |
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| Ort Quelle: | Basel : MDPI, 2012 |
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| Jahr Quelle: | 2021 |
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| Band/Heft Quelle: | 10(2021,1) Artikel-Nummer 152, 14 Seiten |
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| ISSN Quelle: | 2073-4409 |
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| Abstract: | NY-ESO-1-specific T cells have shown promising activity in the treatment of soft tissue sarcoma (STS). However, standardized protocols for their generation are limited. Particularly, cost-effectiveness considerations of cell production protocols are of importance for conducting clinical studies. In this study, two different NY-ESO-1-specific T cell production protocols were compared. Major differences between protocols 1 and 2 include culture medium, interleukin-2 and retronectin concentrations, T cell activation strategy, and the transduction process. NY-ESO-1-specific T cells generated according to the two protocols were investigated for differences in cell viability, transduction efficiency, T cell expansion, immunophenotype as well as functionality. NY-ESO-1-specific T cells showed similar viability and transduction efficiency between both protocols. Protocol 1 generated higher absolute numbers of NY-ESO-1-specific T cells. However, there was no difference in absolute numbers of NY-ESO-1-specific T cell subsets with less-differentiated phenotypes accounting for efficient in vivo expansion and engraftment. Furthermore, cells generated according to protocol 1 displayed higher capacity of TNF-α generation, but lower cytotoxic capacities. Overall, both protocols provided functional NY-ESO-1-specific T cells. However, compared to protocol 1, protocol 2 is advantageous in terms of cost-effectiveness. Cell production protocols should be designed diligently to achieve a cost-effective cellular product for further clinical evaluation. |
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| DOI: | doi:10.3390/cells10010152 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.3390/cells10010152 |
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| | Volltext: https://www.mdpi.com/2073-4409/10/1/152 |
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| | DOI: https://doi.org/10.3390/cells10010152 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | adoptive cell therapy |
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| | cell production protocols |
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| | NY-ESO-1-specific T cells |
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| K10plus-PPN: | 1750417308 |
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| Verknüpfungen: | → Zeitschrift |
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Evaluation of production protocols for the generation of NY-ESO-1-specific T cells / Gong, Wenjie [VerfasserIn]; 14 January 2021 (Online-Ressource)
