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Status: Bibliographieeintrag

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Verfasst von:Gong, Wenjie [VerfasserIn]   i
 Wang, Lei [VerfasserIn]   i
 Stock, Sophia [VerfasserIn]   i
 Ni, Ming [VerfasserIn]   i
 Schubert, Maria-Luisa [VerfasserIn]   i
 Neuber, Brigitte [VerfasserIn]   i
 Kleist, Christian [VerfasserIn]   i
 Hückelhoven-Krauss, Angela [VerfasserIn]   i
 Wu, Depei [VerfasserIn]   i
 Müller-Tidow, Carsten [VerfasserIn]   i
 Schmitt, Anita [VerfasserIn]   i
 Shiku, Hiroshi [VerfasserIn]   i
 Schmitt, Michael [VerfasserIn]   i
 Sellner, Leopold [VerfasserIn]   i
Titel:Evaluation of production protocols for the generation of NY-ESO-1-specific T cells
Verf.angabe:Wenjie Gong, Lei Wang, Sophia Stock, Ming Ni, Maria-Luisa Schubert, Brigitte Neuber, Christian Kleist, Angela Hückelhoven-Krauss, Depei Wu, Carsten Müller-Tidow, Anita Schmitt, Hiroshi Shiku, Michael Schmitt and Leopold Sellner
E-Jahr:2021
Jahr:14 January 2021
Fussnoten:Gesehen am 04.03.2021
Titel Quelle:Enthalten in: Cells
Ort Quelle:Basel : MDPI, 2012
Jahr Quelle:2021
Band/Heft Quelle:10(2021,1) Artikel-Nummer 152, 14 Seiten
ISSN Quelle:2073-4409
Abstract:NY-ESO-1-specific T cells have shown promising activity in the treatment of soft tissue sarcoma (STS). However, standardized protocols for their generation are limited. Particularly, cost-effectiveness considerations of cell production protocols are of importance for conducting clinical studies. In this study, two different NY-ESO-1-specific T cell production protocols were compared. Major differences between protocols 1 and 2 include culture medium, interleukin-2 and retronectin concentrations, T cell activation strategy, and the transduction process. NY-ESO-1-specific T cells generated according to the two protocols were investigated for differences in cell viability, transduction efficiency, T cell expansion, immunophenotype as well as functionality. NY-ESO-1-specific T cells showed similar viability and transduction efficiency between both protocols. Protocol 1 generated higher absolute numbers of NY-ESO-1-specific T cells. However, there was no difference in absolute numbers of NY-ESO-1-specific T cell subsets with less-differentiated phenotypes accounting for efficient in vivo expansion and engraftment. Furthermore, cells generated according to protocol 1 displayed higher capacity of TNF-α generation, but lower cytotoxic capacities. Overall, both protocols provided functional NY-ESO-1-specific T cells. However, compared to protocol 1, protocol 2 is advantageous in terms of cost-effectiveness. Cell production protocols should be designed diligently to achieve a cost-effective cellular product for further clinical evaluation.
DOI:doi:10.3390/cells10010152
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext ; Verlag: https://doi.org/10.3390/cells10010152
 Volltext: https://www.mdpi.com/2073-4409/10/1/152
 DOI: https://doi.org/10.3390/cells10010152
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:adoptive cell therapy
 cell production protocols
 NY-ESO-1-specific T cells
K10plus-PPN:1750417308
Verknüpfungen:→ Zeitschrift

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