The role of CAP3 in CD95 signaling

• Verfasst von: Golks, Alexander [VerfasserIn]
• Verfasst von: Krammer, Peter H. [VerfasserIn]
• Verfasst von: Lavrik, Inna N. [VerfasserIn]
• Titel: The role of CAP3 in CD95 signaling
• Titelzusatz: new insights into the mechanism of procaspase-8 activation
• Verf.angabe: A. Golks, D. Brenner, I. Schmitz, C. Watzl, A. Krueger, P.H. Krammer and I.N. Lavrik
• Jahr: 2006
• Jahr des Originals: 2005
• Umfang: 10 S.
• Teil: volume:13
• Teil: year:2006
• Teil: number:3
• Teil: pages:489-498
• Teil: extent:10
• Fussnoten: Published: 23 September 2005 ; Gesehen am 04.03.2021
• Titel Quelle: Enthalten in: Cell death and differentiation
• Ort Quelle: Houndmills, Basingstoke : Nature Publishing Group, 1997
• Jahr Quelle: 2006
• Band/Heft Quelle: 13(2006), 3, Seite 489-498
• ISSN Quelle: 1476-5403
• DOI: doi:10.1038/sj.cdd.4401766
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1750419610

Abstract

Formation of the CD95 (APO-1/Fas) death inducing signaling complex (DISC) plays a central role in CD95 signaling. Previously, CD95 DISC composition was analyzed by two-dimensional gel electrophoresis and four major cytotoxicity-associated proteins (CAP1-4) were found. CAP1 and CAP2 were defined to be unmodified and phosphorylated FADD, respectively. CAP4 was identified as procaspase-8a. CAP3, however, has remained elusive. In this study, we demonstrate that CAP3 is an intermediate of procaspase-8 processing. CAP3 is generated within seconds of DISC formation and subsequently processed to the prodomain of procaspase-8a that is known as p26 (CAP5). These findings lead to new insights into the mechanism of procaspase-8 processing and apoptosis initiation.