NF-κB synergizes with NF-AT and NF-IL6 in activation of the IL-4 gene in T cells

• Verfasst von: Li-Weber, Min [VerfasserIn]
• Verfasst von: Giaisi, Marco [VerfasserIn]
• Verfasst von: Baumann, Sven [VerfasserIn]
• Verfasst von: Pálfi, Katalin [VerfasserIn]
• Verfasst von: Krammer, Peter H. [VerfasserIn]
• Titel: NF-κB synergizes with NF-AT and NF-IL6 in activation of the IL-4 gene in T cells
• Verf.angabe: Min Li‐Weber, Marco Giaisi, Sven Baumann, Katalin Pálfi and Peter H. Krammer
• E-Jahr: 2004
• Jahr: 23 March 2004
• Umfang: 8 S.
• Teil: volume:34
• Teil: year:2004
• Teil: number:4
• Teil: pages:1111-1118
• Teil: extent:8
• Fussnoten: Gesehen am 04.03.2021
• Titel Quelle: Enthalten in: European journal of immunology
• Ort Quelle: Weinheim : Wiley-VCH, 1971
• Jahr Quelle: 2004
• Band/Heft Quelle: 34(2004), 4, Seite 1111-1118
• ISSN Quelle: 1521-4141
• DOI: doi:10.1002/eji.200324687
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Cytokine
• Sach-SW: T lymphocyte
• Sach-SW: Transcription factor
• K10plus-PPN: 1750443627

Abstract

IL-4 plays a pivotal role in the development of the Th2 cell mediated humoral immune response and causes IgE-dependent allergic inflammatory diseases. Expression of IL-4 in differentiated Th2 cells is regulated by transcription factors such as NF-AT, AP-1 and NF-IL6. Recently, increasing evidence indicates that the pro-inflammatory transcription factor NF-κB may also participate inIL-4 expression. In this study, we show that the IL-4 promoter is synergistically activated by NF-κB, NF-AT and NF-IL6 at the NF-κB/NF-AT/NF-IL6 composite sites. In addition, we performed the chromatin immunoprecipitation technique to determine the functional relevance of NF-κB in the activation of the IL-4 gene in vivo. We demonstrate that NF-κB binds to the IL-4 promoter in vivo upon T cell activation. Inhibition of NF-κB nuclear translocation in living cells blocked binding of NF-κB to the IL-4 promoter. The data provide first evidence that NF-κB is directly involved in IL-4 transcription.