Effect of low ionic strength on anti-Pr reactions

• Verfasst von: Leo, Albrecht [VerfasserIn]
• Verfasst von: Kreft, Heidi [VerfasserIn]
• Verfasst von: Hack, Hildegard [VerfasserIn]
• Verfasst von: Roelcke, Dieter [VerfasserIn]
• Titel: Effect of low ionic strength on anti-Pr reactions
• Verf.angabe: A. Leo, H. Kreft, H. Hack, D. Roelcke
• Jahr: 1996
• Umfang: 4 S.
• Teil: volume:33
• Teil: year:1996
• Teil: pages:22-25
• Teil: extent:4
• Titel Quelle: Enthalten in: Beiträge zur Infusionstherapie und Transfusionsmedizin
• Ort Quelle: Basel [u.a.] : Karger, 1994
• Jahr Quelle: 1996
• Band/Heft Quelle: 33(1996), Seite 22-25
• Sprache: eng
• Sach-SW: Agglutinins
• Sach-SW: Antigen-Antibody Reactions
• Sach-SW: Blood Group Antigens
• Sach-SW: Cryoglobulins
• Sach-SW: Humans
• Sach-SW: Isoantigens
• Sach-SW: Osmolar Concentration
• Sach-SW: Temperature
• K10plus-PPN: 1750536889

Abstract

The effect of low ionic strength (LIS) on 28 anti-Pr, 20 anti-I and 20 anti-i cold agglutinins was investigated. The reaction of the anti-Pr CAs varies markedly. In most cases LIS has an enhancing effect. In some cases the thermal amplitude was widened so far that the reaction at 37 degrees C under LIS was stronger than at 0 degree C in PBS. With regard to the anti-Pr subspecificities anti Pr1, -Pr2 and -Pr3 or to the distinction of the immunodominant NeuNAc group (alpha a2,6- or alpha 2,3-bond) a correlation between these characteristics and the reaction in LIS could not be identified. The anti-I are not influenced by LISS, anti-i in a few cases. The reason for the variable reaction of the anti-Pr remains unclear. To further elucidate the LISS effect on anti-Pr, the contribution of the antibody structure should be regarded, but data for the use of H- and L-chain genes in anti-Pr are sparse. For compatibility testing in the routine laboratory, LISS-sensitive anti-Pr may play a role in disturbing the screening for RBC antibodies.