Production of antibodies to the constant region of the mouse T-cell-receptor β-chain

• Verfasst von: Schenkel, Johannes [VerfasserIn]
• Verfasst von: Schäfer, Hubert [VerfasserIn]
• Verfasst von: Koch, Susanne [VerfasserIn]
• Verfasst von: Burger, Reinhard [VerfasserIn]
• Titel: Production of antibodies to the constant region of the mouse T-cell-receptor β-chain
• Verf.angabe: Johannes Schenkel, Hubert Schäfer, Susanne Koch, Reinhard Burger
• Jahr: 1991
• Umfang: 4 S.
• Teil: volume:99
• Teil: year:1991
• Teil: number:1-6
• Teil: pages:99-102
• Teil: extent:4
• Fussnoten: Gesehen am 05.03.2021
• Titel Quelle: Enthalten in: Acta pathologica, microbiologica et immunologica Scandinavica
• Ort Quelle: Oxford : Wiley-Blackwell, 1988
• Jahr Quelle: 1991
• Band/Heft Quelle: 99(1991), 1-6, Seite 99-102
• ISSN Quelle: 1600-0463
• DOI: doi:10.1111/j.1699-0463.1991.tb05126.x
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: expression vector
• Sach-SW: T lymphocytes
• Sach-SW: T-cell receptor
• K10plus-PPN: 1750569248

Abstract

To generate antibodies to the constant region of the mouse T-cell-receptor β chain, the corresponding cDNA clone 86T5 was subcloned into the bacterial expression vector pEX2. The induced hybrid protein consisting of a 30 kDa T-cell-receptor segment and a β-galactosidase carrier moiety was used for immunization. In Western blots using lysates of a T-cell-receptor-positive T-cell hybrid, the antisera obtained reacted with a 42 kDa protein under reducing and a 85 kDa protein under nonreducing conditions. The antisera did not react in binding assays with intact T lymphocytes, i.e. membrane-associated T-cell-receptor protein.