Cyclosporin A

• Verfasst von: Nebl, Gabriele [VerfasserIn]
• Verfasst von: Meuer, Stefan [VerfasserIn]
• Verfasst von: Samstag, Yvonne [VerfasserIn]
• Titel: Cyclosporin A
• Titelzusatz: resistant transactivation of the IL-2 promoter requires activity of okadaic acid-sensitive aerine/threonine phosphatases
• Verf.angabe: Gabriele Nebl, Stefan C. Meuer and Yvonne Samstag
• Jahr: 1998
• Umfang: 8 S.
• Teil: volume:161
• Teil: year:1998
• Teil: number:4
• Teil: pages:1803-1810
• Teil: extent:8
• Fussnoten: Published online: August 15, 1998 ; Gesehen am 08.03.2021
• Titel Quelle: Enthalten in: The journal of immunology
• Ort Quelle: Bethesda, Md. : Soc., 1916
• Jahr Quelle: 1998
• Band/Heft Quelle: 161(1998), 4, Seite 1803-1810
• ISSN Quelle: 1550-6606
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1750752905

Abstract

Expression of the IL-2 gene requires activation of T cells through stimulation of the TCR and costimulation through accessory receptors. We have found recently that okadaic acid-sensitive Ser/Thr phosphatases are involved in a cyclosporin A-insensitive pathway that selectively transmits costimulatory signals. In this study, we analyzed whether activities of these phosphatases are necessary for the expression of the IL-2 gene. In both activated peripheral blood T lymphocytes and activated tumorigenic T cell lines, IL-2 gene expression was blocked at the transcriptional level by okadaic acid. The transcription factors active at the IL-2 promoter were differentially influenced: upon down-modulation of okadaic acid-sensitive phosphatases, transactivation by octamer, NF-κB, and NF of activated T cells proteins was abrogated, while transactivation by AP-1 proteins was even enhanced.