Bisulfite-free epigenomics and genomics of single cells through methylation-sensitive restriction

• Verfasst von: Niemöller, Christoph [VerfasserIn]
• Verfasst von: Plass, Christoph [VerfasserIn]
• Verfasst von: Lutsik, Pavlo [VerfasserIn]
• Verfasst von: Lipka, Daniel [VerfasserIn]
• Titel: Bisulfite-free epigenomics and genomics of single cells through methylation-sensitive restriction
• Verf.angabe: Christoph Niemöller, Julius Wehrle, Julian Riba, Rainer Claus, Nathalie Renz, Janika Rhein, Sabine Bleul, Juliane M. Stosch, Justus Duyster, Christoph Plass, Pavlo Lutsik, Daniel B. Lipka, Michael Lübbert & Heiko Becker
• E-Jahr: 2021
• Jahr: 01 February 2021
• Umfang: 10 S.
• Fussnoten: Gesehen am 26.11.2021
• Titel Quelle: Enthalten in: Communications biology
• Ort Quelle: London : Springer Nature, 2018
• Jahr Quelle: 2021
• Band/Heft Quelle: 4(2021), Artikel-ID 153, Seite 1-10
• ISSN Quelle: 2399-3642
• DOI: doi:10.1038/s42003-021-01661-w
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1751172015

Abstract

Single-cell multi-omics are powerful means to study cell-to-cell heterogeneity. Here, we present a single-tube, bisulfite-free method for the simultaneous, genome-wide analysis of DNA methylation and genetic variants in single cells: epigenomics and genomics of single cells analyzed by restriction (epi-gSCAR). By applying this method, we obtained DNA methylation measurements of up to 506,063 CpGs and up to 1,244,188 single-nucleotide variants from single acute myeloid leukemia-derived cells. We demonstrate that epi-gSCAR generates accurate and reproducible measurements of DNA methylation and allows to differentiate between cell lines based on the DNA methylation and genetic profiles.