Beyond cell death

• Verfasst von: Köhler, Bruno Christian [VerfasserIn]
• Verfasst von: Scherr, Anna-Lena [VerfasserIn]
• Verfasst von: Lorenz, Stephan [VerfasserIn]
• Verfasst von: Urbanik, Toni [VerfasserIn]
• Verfasst von: Kautz, Nicole [VerfasserIn]
• Verfasst von: Elßner, Christin [VerfasserIn]
• Verfasst von: Welte, Stefan Ezechiel [VerfasserIn]
• Verfasst von: Lorenzo Bermejo, Justo [VerfasserIn]
• Verfasst von: Jäger, Dirk [VerfasserIn]
• Verfasst von: Schulze-Bergkamen, Henning [VerfasserIn]
• Titel: Beyond cell death
• Titelzusatz: antiapoptotic Bcl-2 proteins regulate migration and invasion of colorectal cancer cells in vitro
• Verf.angabe: Bruno Christian Koehler, Anna-Lena Scherr, Stephan Lorenz, Toni Urbanik, Nicole Kautz, Christin Elssner, Stefan Welte, Justo Lorenzo Bermejo, Dirk Jäger, Henning Schulze-Bergkamen
• E-Jahr: 2013
• Jahr: October 3, 2013
• Umfang: 12 S.
• Fussnoten: Gesehen am 15.12.2021
• Titel Quelle: Enthalten in: PLOS ONE
• Ort Quelle: San Francisco, California, US : PLOS, 2006
• Jahr Quelle: 2013
• Band/Heft Quelle: 8(2013), 10, Artikel-ID e76446, Seite 1-12
• ISSN Quelle: 1932-6203
• DOI: doi:10.1371/journal.pone.0076446
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Apoptosis
• Sach-SW: Cancer cell migration
• Sach-SW: Cell death
• Sach-SW: Colorectal cancer
• Sach-SW: HT29 cells
• Sach-SW: Small interfering RNA
• Sach-SW: SW480 cells
• Sach-SW: Transfection
• K10plus-PPN: 1752055152

Abstract

Migration and invasion of malignant cells are prerequisites for cancer progression and metastasis. The Bcl-2 family of proteins consists of about 25 members and has been extensively studied in the context of apoptosis. Despite the fact that small molecules targeting Bcl-2 proteins have already entered clinical trials, very few studies investigated a role of antiapoptotic Bcl-2 proteins beside cell death in the context of metastasis. The aim of this study was to dissect a potential role of the antiapoptotic Bcl-2 proteins Mcl-1, Bcl-2 and Bcl-xL on migration and invasion of colorectal cancer cells independent of their cell death control function. We used migration and invasion assays as well as three dimensional cell cultures to analyze colorectal cancer cell lines (HT29 and SW480) after siRNA mediated knockdown or overexpression of Mcl-1, Bcl-2 or Bcl-xL. We observed neither spontaneous cell death induction nor impaired proliferation of cells lacking Mcl-1, Bcl-2 or Bcl-xL. In contrast, knockdown of Mcl-1 led to increased proliferation. Strikingly, we demonstrate a profound impairment of both, migration and invasion, of colorectal cancer cells after Mcl-1, Bcl-2 or Bcl-xL knockdown. This phenotype was completely revised in cells overexpressing Mcl-1, Bcl-2 or Bcl-xL. The most pronounced effect among the investigated proteins was observed for Bcl-2. The data presented indicate a pivotal role of Mcl-1, Bcl-2 and Bcl-xL for migration and invasion of colorectal cancer cells independent of their known antiapoptotic effects. Thus, our study illustrates novel antitumoral mechanisms of Bcl-2 protein targeting.