Single-nucleotide polymorphisms of MMP-2 gene in stroke subtypes

• Verfasst von: Fatar, Marc [VerfasserIn]
• Verfasst von: Stroick, Mark Gregor [VerfasserIn]
• Verfasst von: Steffens, Michael [VerfasserIn]
• Verfasst von: Senn, Eva Lore [VerfasserIn]
• Verfasst von: Reuter, Björn [VerfasserIn]
• Verfasst von: Bukow, Simone [VerfasserIn]
• Verfasst von: Griebe, Martin [VerfasserIn]
• Verfasst von: Alonso, Angelika [VerfasserIn]
• Verfasst von: Lichtner, Peter [VerfasserIn]
• Verfasst von: Bugert, Peter [VerfasserIn]
• Verfasst von: Meitinger, Thomas [VerfasserIn]
• Verfasst von: Wienker, Thomas F. [VerfasserIn]
• Verfasst von: Hennerici, Michael G. [VerfasserIn]
• Titel: Single-nucleotide polymorphisms of MMP-2 gene in stroke subtypes
• Verf.angabe: Marc Fatar, Mark Stroick, Michael Steffens, Eva Senn, Bjoern Reuter, Simone Bukow, Martin Griebe, Angelika Alonso, Peter Lichtner, Peter Bugert, Thomas Meitinger, Thomas F. Wienker, Michael G. Hennerici
• E-Jahr: 2008
• Jahr: June 17, 2008
• Umfang: 7 S.
• Fussnoten: Gesehen am 26.03.2021
• Titel Quelle: Enthalten in: Cerebrovascular diseases
• Ort Quelle: Basel : Karger, 1991
• Jahr Quelle: 2008
• Band/Heft Quelle: 26(2008), 2, Seite 113-119
• ISSN Quelle: 1421-9786
• DOI: doi:10.1159/000139657
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1752580834

Abstract

<i>Background:</i> Matrix metalloproteinases (MMP) are expressed after ischemic stroke. These proteases are responsible for a higher incidence of hemorrhages, are correlated to size of infarction and influence the effects of recombinant tissue plasminogen activator treatment. We therefore evaluated single nucleotide polymorphisms (SNP) of MMP-2 in different subtypes of stroke patients in an association study using a case-control design. <i>Methods:</i> 197 stroke patients were divided according to modified TOAST criteria (small vessel disease, large vessel disease, hemorrhagic stroke and asymptomatic carotid artery stenosis) and compared to 143 controls. Clinical data like age, sex, risk factors and diagnostic results including MRI or cranial CT scans and ultrasound evaluations of intra- and extracranial arteries were obtained. Genotypes of MMP-2 (12 SNP) were compared to controls and DNA samples were analyzed by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) analysis. Logistic regression analysis was performed for small vessel disease to test for interactions between markers and defined clinical risk factors. Additionally, MMP-2 serum levels obtained in the first 24 h after stroke were measured. <i>Results:</i> From the MMP-2 gene, 5 markers (rs1030868, rs2241145, rs2287074, rs2287076, rs7201) showed a significant association with small vessel infarcts (p < 0.05) and rs7201:g.C was identified as an independent risk factor by multivariable logistic regression analysis. MMP-2 protein levels were significantly lower in this group (174 ± 48 ng/dl) versus controls (214 ± 56 ng/dl). For other stroke subtypes, no significant association with MMP-2 SNP could be found. <i>Conclusion:</i> Our study demonstrates an association of the MMP-2 gene with the development of lacunar stroke, and no association of MMP-2 with other stroke subtypes.