| |  Online-Ressource |
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| Verfasst von: | Delacher, Michael [VerfasserIn]  |
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| | Barra, Melanie M. [VerfasserIn] |
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| | Herzig, Yonatan [VerfasserIn] |
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| | Eichelbaum, Katrin [VerfasserIn] |
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| | Rafiee, Mahmoud-Reza [VerfasserIn] |
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| | Richards, David [VerfasserIn] |
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| | Träger, Ulrike [VerfasserIn] |
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| | Hofer, Ann-Cathrin [VerfasserIn] |
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| | Kazakov, Alexander [VerfasserIn] |
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| | Braband, Kathrin L. [VerfasserIn] |
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| | Gonzalez, Marina [VerfasserIn] |
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| | Wöhrl, Lukas [VerfasserIn] |
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| | Schambeck, Kathrin [VerfasserIn] |
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| | Imbusch, Charles [VerfasserIn] |
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| | Abramson, Jakub [VerfasserIn] |
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| | Krijgsveld, Jeroen [VerfasserIn] |
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| | Feuerer, Markus [VerfasserIn] |
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| Titel: | Quantitative proteomics identifies TCF1 as a negative regulator of Foxp3 expression in conventional T cells |
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| Verf.angabe: | Michael Delacher, Melanie M. Barra, Yonatan Herzig, Katrin Eichelbaum, Mahmoud-Reza Rafiee, David M. Richards, Ulrike Träger, Ann-Cathrin Hofer, Alexander Kazakov, Kathrin L. Braband, Marina Gonzalez, Lukas Wöhrl, Kathrin Schambeck, Charles D. Imbusch, Jakub Abramson, Jeroen Krijgsveld, and Markus Feuerer |
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| E-Jahr: | 2020 |
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| Jahr: | May 22, 2020 |
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| Umfang: | 15 S. |
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| Fussnoten: | Gesehen am 29.03.2021 |
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| Titel Quelle: | Enthalten in: iScience |
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| Ort Quelle: | Amsterdam : Elsevier, 2018 |
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| Jahr Quelle: | 2020 |
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| Band/Heft Quelle: | 23(2020), 5 vom: Mai, Artikel-ID 101127, Seite 1-15 |
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| ISSN Quelle: | 2589-0042 |
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| Abstract: | Regulatory T cells are important regulators of the immune system and have versatile functions for the homeostasis and repair of tissues. They express the forkhead box transcription factor Foxp3 as a lineage-defining protein. Negative regulators of Foxp3 expression are not well understood. Here, we generated double-stranded DNA probes complementary to the Foxp3 promoter sequence and performed a pull-down with nuclear protein in vitro, followed by elution of bound proteins and quantitative mass spectrometry. Of the Foxp3-promoter-binding transcription factors identified with this approach, one was T cell factor 1 (TCF1). Using viral over-expression, we identified TCF1 as a repressor of Foxp3 expression. In TCF1-deficient animals, increased levels of Foxp3intermediateCD25negative T cells were identified. CRISPR-Cas9 knockout studies in primary human and mouse conventional CD4 T (Tconv) cells revealed that TCF1 protects Tconv cells from inadvertent Foxp3 expression. Our data implicate a role of TCF1 in suppressing Foxp3 expression in activated T cells. |
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| DOI: | doi:10.1016/j.isci.2020.101127 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1016/j.isci.2020.101127 |
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| | Volltext: https://www.sciencedirect.com/science/article/pii/S2589004220303126 |
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| | DOI: https://doi.org/10.1016/j.isci.2020.101127 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Immunology |
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| | Molecular Biology |
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| | Molecular Mechanism of Gene Regulation |
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| | Proteomics |
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| K10plus-PPN: | 1752657373 |
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| Verknüpfungen: | → Zeitschrift |
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Quantitative proteomics identifies TCF1 as a negative regulator of Foxp3 expression in conventional T cells / Delacher, Michael [VerfasserIn]; May 22, 2020 (Online-Ressource)
