| Online-Ressource |
Verfasst von: | Kittel, Maximilian [VerfasserIn]  |
| Thiaucourt, Margot [VerfasserIn]  |
| Gerhards, Catharina [VerfasserIn]  |
| Haselmann, Verena [VerfasserIn]  |
| Neumaier, Michael [VerfasserIn]  |
Titel: | Clinical evaluation of commercial automated SARS-CoV-2 immunoassays |
Verf.angabe: | Maximilian Kittel, Maria Christina Muth, Ingrid Zahn, Heinz-Jürgen Roth, Margot Thiaucourt, Catharina Gerhards, Verena Haselmann, Michael Neumaier, Peter Findeisen |
E-Jahr: | 2021 |
Jahr: | February 2021 |
Umfang: | 7 S. |
Fussnoten: | Gesehen am 31.03.2021 |
Titel Quelle: | Enthalten in: International journal of infectious diseases |
Ort Quelle: | Amsterdam [u.a.] : Elsevier, 1997 |
Jahr Quelle: | 2021 |
Band/Heft Quelle: | 103(2021), Seite 590-596 |
ISSN Quelle: | 1878-3511 |
Abstract: | Objective - Numerous immunoassays for detecting antibodies directed against SARS-CoV-2 have been rapidly developed and released. Validations of these have been performed with a limited number of samples. The lack of standardisation might lead to significantly different results. This study compared ten automated assays from six vendors in terms of sensitivity, specificity and reproducibility. - Methods - This study compared ten fully automated immunoassays from the following vendors: Diasorin, Epitope Diagnostics, Euroimmun, Roche, YHLO, and Snibe. The retrospective part of the study included patients with a laboratory-confirmed COVID-19 infection, and controls comprised patients with a suspected infection, in whom the disease was excluded. Furthermore, biobanked sera were taken as negative controls (n = 97). The retrospective part involved four groups: (1) laboratory-confirmed COVID-19 infection (n = 183); (1B) suspected COVID-19 infection (n = 167) without a qRT-PCR result but positive serological results from at least two different assays, and suspected COVID-19 infection due to a positive serological result from the Roche assay (n = 295); (2) biobanked sera obtained from patients before the emergence of SARS-CoV-2 (n = 97) as negative controls; and (2A) probably COVID-19-negative sera with negative serological results from at least two different assays (n = 152). - Results - Overall diagnostic sensitivities were: Euroimmun (IgA) 87%; Epitope Diagnostics (IgG) 83%; YHLO (IgG) 77%; Roche (IgM/IgG) 77%; Euroimmun (IgG) 75%; Diasorin (IgG) 53%; Epitope Diagnostics (IgM) 52%; Snibe (IgG) 47%; YHLO (IgM) 35%; and Snibe (IgM) 26%. Diagnostic specificities were: YHLO (IgG) 100%; Roche, 100%; Snibe (IgM/IgG) 100%; Diasorin (IgG) 97%; Euroimmun (IgG) 94%; YHLO (IgM) 94%; Euroimmun (IgA) 83%. - Conclusion - Assays from different vendors substantially varied in terms of their performance. These findings might facilitate selection of appropriate serological assays. |
DOI: | doi:10.1016/j.ijid.2020.12.003 |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1016/j.ijid.2020.12.003 |
| Volltext: https://www.sciencedirect.com/science/article/pii/S1201971220325261 |
| DOI: https://doi.org/10.1016/j.ijid.2020.12.003 |
Datenträger: | Online-Ressource |
Sprache: | eng |
Sach-SW: | Automated SARS-CoV-2 antibody detection |
| High throughpu testing |
| Longitudional monitoring of antibody development |
| Pandemic control |
| Seroconversion |
| Serpprevalence |
K10plus-PPN: | 1752963865 |
Verknüpfungen: | → Zeitschrift |
Clinical evaluation of commercial automated SARS-CoV-2 immunoassays / Kittel, Maximilian [VerfasserIn]; February 2021 (Online-Ressource)