Expression of CD69 on T-cells from HIV-1-infected children and adolescents increases with increasing viral load

• Verfasst von: Böhler, Thomas [VerfasserIn]
• Verfasst von: Walcher, Judith [VerfasserIn]
• Verfasst von: Hölzl-Wenig, Gabriele [VerfasserIn]
• Verfasst von: Schnitzler, Paul [VerfasserIn]
• Verfasst von: Geiss, M. [VerfasserIn]
• Verfasst von: Buchholz, Bernd [VerfasserIn]
• Verfasst von: Linde, R. [VerfasserIn]
• Verfasst von: Rütschle, H. [VerfasserIn]
• Verfasst von: Debatin, Klaus-Michael [VerfasserIn]
• Titel: Expression of CD69 on T-cells from HIV-1-infected children and adolescents increases with increasing viral load
• Verf.angabe: T. Böhler, J. Walcher, G. Hölzl-Wenig, P. Schnitzler, M. Geiss, B. Buchholz, R. Linde, H. Rütschle, K.M. Debatin
• E-Jahr: 1999
• Jahr: July 1999
• Umfang: 7 S.
• Fussnoten: Gesehen am 09.04.2021
• Titel Quelle: Enthalten in: European journal of pediatrics
• Ort Quelle: Berlin : Springer Science & Business Media B.V., 1975
• Jahr Quelle: 1999
• Band/Heft Quelle: 158(1999), 8, Seite 638-644
• ISSN Quelle: 1432-1076
• DOI: doi:10.1007/s004310051167
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Adolescent
• Sach-SW: Antigens, CD
• Sach-SW: Child
• Sach-SW: Child, Preschool
• Sach-SW: Evaluation Studies as Topic
• Sach-SW: Female
• Sach-SW: Flow Cytometry
• Sach-SW: HIV Infections
• Sach-SW: HIV-1
• Sach-SW: Humans
• Sach-SW: Male
• Sach-SW: T-Lymphocytes
• Sach-SW: Viral Load
• K10plus-PPN: 1753570581

Abstract

We evaluated the use of a whole-blood assay that measures spontaneous and activation-induced CD69 expression on peripheral blood T-cells in vitro for assessment of T-cell function in HIV-1-infected paediatric patients. Heparinized venous blood from 28 HIV-1 positive children and adolescents and 23 healthy controls was incubated for 4 h with or without 5 microg/ml phytohaemagglutinin (PHA). Thereafter, analysis of CD69 expression on CD4+ and CD8+ T-cells was done by flow cytometry; simultaneously we determined CD4+ T-cell counts and plasma HIV-1 viral load. Neither spontaneous nor PHA-induced CD69 expression differed significantly between HIV-1 positive patients and healthy controls. However, T-cells from HIV-1 positive patients with plasma HIV-1 viral load levels above 70x10(3) copies/ml showed a higher spontaneous CD69 expression than T-cells from patients with lower plasma viral load levels in different stages of the disease. Antiretroviral treatment in four patients reduced spontaneous CD69 expression in CD4+ T-cells and PHA-induced CD69 expression in CD4+ and CD8+ T-cells significantly after 8 weeks of therapy. Conclusion Spontaneous and activation-induced expression of the early (activation) antigen CD69 on peripheral blood T-cells does not distinguish HIV-1 positive patients from HIV-1 negative healthy controls and is not correlated with peripheral blood CD4+ T-cell counts. This test may not be a reliable marker for functional T-cell deficiency during early stages of HIV disease. Increased spontaneous as well as PHA-induced CD69 expression on T-cells from HIV-1-infected children and adolescents in vitro may rather reflect HIV-induced pre-activation of T-cells in vivo.