| |  Online-Ressource |
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| Verfasst von: | Storch-Hagenlocher, Brigitte [VerfasserIn]  |
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| | Haas, Jürgen [VerfasserIn] |
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| | Vogt-Schaden, Maria-Elisabeth [VerfasserIn] |
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| | Bentz, Martin [VerfasserIn] |
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| | Hoffmann, Lisa Ann [VerfasserIn] |
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| | Biessmann, Annette [VerfasserIn] |
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| | Wildemann, Brigitte [VerfasserIn] |
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| Titel: | Molecular analysis of the CDR3 encoding region of the immunoglobulin heavy chain locus in cerebrospinal fluid cells as a diagnostic tool in lymphomatous meningitis |
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| Verf.angabe: | Brigitte Storch‐Hagenlocher, Jürgen Haas, Maria Elisabeth Vogt‐Schaden, Martin Bentz, Lisa Ann Hoffmann, Annette Biessmann, and Brigitte Wildemann |
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| Jahr: | 2000 |
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| Umfang: | 7 S. |
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| Teil: | volume:47 |
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| | year:2000 |
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| | number:2 |
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| | pages:211-217 |
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| | extent:7 |
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| Fussnoten: | Elektronische Reproduktion der Druck-Ausgabe ; Gesehen am 12.04.2021 |
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| Titel Quelle: | Enthalten in: Annals of neurology |
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| Ort Quelle: | Hoboken, NJ : Wiley-Blackwell, 1977 |
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| Jahr Quelle: | 2000 |
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| Band/Heft Quelle: | 47(2000), 2, Seite 211-217 |
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| ISSN Quelle: | 1531-8249 |
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| Abstract: | The diagnosis of leptomeningeal B-cell lymphoma is based on the identification of malignant B cells in the cerebrospinal fluid (CSF). Frequently, cytology does not allow clear distinction between neoplastic lymphoid cells and reactively transformed mononuclear cells. Individual B-cell clones can be identified on the basis of DNA sequences that encode the highly diverse complementarity-determining region (CDR3) of the immunoglobulin heavy chain locus (IgH). We studied CSF samples from 5 patients with B-cell malignancies and cytological evidence of leptomeningeal involvement, using polymerase chain reaction (PCR)-based high-resolution capillary electrophoresis and automated fluorescence analysis to detect PCR fragments. As controls, we assessed CSF specimens from 7 patients with inflammatory neurological diseases and three samples without pathological findings. In all patients with B-cell malignancies, a single PCR product was generated, indicating that CDR3-specific fragments were derived from monoclonal cell populations. CSF samples from patients with inflammatory diseases yielded multiple CDR3 amplicons, suggesting the presence of a polyclonal B-cell activation. No PCR product could be amplified in normal CSF samples. Automated fluorescence detection of CDR3 fragments is a highly sensitive and rapid method to distinguish neoplastic monoclonal and reactive polyclonal B-cell populations in the CSF. Ann Neurol 2000;47:211-217 |
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| DOI: | doi:10.1002/1531-8249(200002)47:2<211::AID-ANA11>3.0.CO;2-9 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1002/1531-8249(200002)47:2<211::AID-ANA11>3.0.CO;2-9 |
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| | Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1002/1531-8249%28200002%2947%3A2%3C211%3A%3AAID-ANA11%3E3.0.CO%3B2-9 |
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| | DOI: https://doi.org/10.1002/1531-8249(200002)47:2<211::AID-ANA11>3.0.CO;2-9 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| K10plus-PPN: | 1753638682 |
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| Verknüpfungen: | → Zeitschrift |
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Molecular analysis of the CDR3 encoding region of the immunoglobulin heavy chain locus in cerebrospinal fluid cells as a diagnostic tool in lymphomatous meningitis / Storch-Hagenlocher, Brigitte [VerfasserIn]; 2000 (Online-Ressource)
