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Verfasst von:Storch-Hagenlocher, Brigitte [VerfasserIn]   i
 Haas, Jürgen [VerfasserIn]   i
 Vogt-Schaden, Maria-Elisabeth [VerfasserIn]   i
 Bentz, Martin [VerfasserIn]   i
 Hoffmann, Lisa Ann [VerfasserIn]   i
 Biessmann, Annette [VerfasserIn]   i
 Wildemann, Brigitte [VerfasserIn]   i
Titel:Molecular analysis of the CDR3 encoding region of the immunoglobulin heavy chain locus in cerebrospinal fluid cells as a diagnostic tool in lymphomatous meningitis
Verf.angabe:Brigitte Storch‐Hagenlocher, Jürgen Haas, Maria Elisabeth Vogt‐Schaden, Martin Bentz, Lisa Ann Hoffmann, Annette Biessmann, and Brigitte Wildemann
Jahr:2000
Umfang:7 S.
Teil:volume:47
 year:2000
 number:2
 pages:211-217
 extent:7
Fussnoten:Elektronische Reproduktion der Druck-Ausgabe ; Gesehen am 12.04.2021
Titel Quelle:Enthalten in: Annals of neurology
Ort Quelle:Hoboken, NJ : Wiley-Blackwell, 1977
Jahr Quelle:2000
Band/Heft Quelle:47(2000), 2, Seite 211-217
ISSN Quelle:1531-8249
Abstract:The diagnosis of leptomeningeal B-cell lymphoma is based on the identification of malignant B cells in the cerebrospinal fluid (CSF). Frequently, cytology does not allow clear distinction between neoplastic lymphoid cells and reactively transformed mononuclear cells. Individual B-cell clones can be identified on the basis of DNA sequences that encode the highly diverse complementarity-determining region (CDR3) of the immunoglobulin heavy chain locus (IgH). We studied CSF samples from 5 patients with B-cell malignancies and cytological evidence of leptomeningeal involvement, using polymerase chain reaction (PCR)-based high-resolution capillary electrophoresis and automated fluorescence analysis to detect PCR fragments. As controls, we assessed CSF specimens from 7 patients with inflammatory neurological diseases and three samples without pathological findings. In all patients with B-cell malignancies, a single PCR product was generated, indicating that CDR3-specific fragments were derived from monoclonal cell populations. CSF samples from patients with inflammatory diseases yielded multiple CDR3 amplicons, suggesting the presence of a polyclonal B-cell activation. No PCR product could be amplified in normal CSF samples. Automated fluorescence detection of CDR3 fragments is a highly sensitive and rapid method to distinguish neoplastic monoclonal and reactive polyclonal B-cell populations in the CSF. Ann Neurol 2000;47:211-217
DOI:doi:10.1002/1531-8249(200002)47:2<211::AID-ANA11>3.0.CO;2-9
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Volltext ; Verlag: https://doi.org/10.1002/1531-8249(200002)47:2<211::AID-ANA11>3.0.CO;2-9
 Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1002/1531-8249%28200002%2947%3A2%3C211%3A%3AAID-ANA11%3E3.0.CO%3B2-9
 DOI: https://doi.org/10.1002/1531-8249(200002)47:2<211::AID-ANA11>3.0.CO;2-9
Datenträger:Online-Ressource
Sprache:eng
K10plus-PPN:1753638682
Verknüpfungen:→ Zeitschrift

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