Status: Bibliographieeintrag
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| Verfasst von: | Rüthnick, Diana [VerfasserIn]  |
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| | Vitale, Jlenia [VerfasserIn] |
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| | Neuner, Annett [VerfasserIn] |
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| | Schiebel, Elmar [VerfasserIn] |
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| Titel: | The N-terminus of Sfi1 and yeast centrin Cdc31 provide the assembly site for a new spindle pole body |
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| Verf.angabe: | Diana Rüthnick, Jlenia Vitale, Annett Neuner, and Elmar Schiebel |
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| E-Jahr: | 2021 |
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| Jahr: | 1 March 2021 |
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| Umfang: | 21 S. |
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| Teil: | volume:220 |
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| | year:2021 |
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| | number:3 |
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| | elocationid:e202004196 |
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| | pages:1-21 |
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| | extent:21 |
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| Fussnoten: | Gesehen am 20.04.2021 |
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| Titel Quelle: | Enthalten in: The journal of cell biology |
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| Ort Quelle: | New York, NY : Rockefeller Univ. Press, 1962 |
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| Jahr Quelle: | 2021 |
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| Band/Heft Quelle: | 220(2021), 3, Artikel-ID e202004196, Seite 1-21 |
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| ISSN Quelle: | 1540-8140 |
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| Abstract: | The spindle pole body (SPB) provides microtubule-organizing functions in yeast and duplicates exactly once per cell cycle. The first step in SPB duplication is the half-bridge to bridge conversion via the antiparallel dimerization of the centrin (Cdc31)-binding protein Sfi1 in anaphase. The bridge, which is anchored to the old SPB on the proximal end, exposes free Sfi1 N-termini (N-Sfi1) at its distal end. These free N-Sfi1 promote in G1 the assembly of the daughter SPB (dSPB) in a yet unclear manner. This study shows that N-Sfi1 including the first three Cdc31 binding sites interacts with the SPB components Spc29 and Spc42, triggering the assembly of the dSPB. Cdc31 binding to N-Sfi1 promotes Spc29 recruitment and is essential for satellite formation. Furthermore, phosphorylation of N-Sfi1 has an inhibitory effect and delays dSPB biogenesis until G1. Taking these data together, we provide an understanding of the initial steps in SPB assembly and describe a new function of Cdc31 in the recruitment of dSPB components. |
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| DOI: | doi:10.1083/jcb.202004196 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1083/jcb.202004196 |
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| | DOI: https://doi.org/10.1083/jcb.202004196 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| K10plus-PPN: | 175471496X |
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| Verknüpfungen: | → Zeitschrift |
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¬The¬ N-terminus of Sfi1 and yeast centrin Cdc31 provide the assembly site for a new spindle pole body / Rüthnick, Diana [VerfasserIn]; 1 March 2021 (Online-Ressource)
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