An unexpected role for FosB in activation-induced cell death of T cells

• Verfasst von: Baumann, Sven [VerfasserIn]
• Verfasst von: Angel, Peter [VerfasserIn]
• Verfasst von: Krammer, Peter H. [VerfasserIn]
• Titel: An unexpected role for FosB in activation-induced cell death of T cells
• Verf.angabe: Sven Baumann, Jochen Hess, Sören T. Eichhorst, Andreas Krueger, Peter Angel, Peter H. Krammer, and Sabine Kirchhoff
• E-Jahr: 2003
• Jahr: 5 March 2003
• Umfang: 7 S.
• Teil: volume:22
• Teil: year:2003
• Teil: number:9
• Teil: pages:1333-1339
• Teil: extent:7
• Fussnoten: Gesehen am 14.04.2021
• Titel Quelle: Enthalten in: Oncogene
• Ort Quelle: London : Springer Nature, 1997
• Jahr Quelle: 2003
• Band/Heft Quelle: 22(2003), 9, Seite 1333-1339
• ISSN Quelle: 1476-5594
• DOI: doi:10.1038/sj.onc.1206126
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1754867294

Abstract

The CD95 (APO-1/Fas) system plays a major role in induction of apoptosis in lymphoid and nonlymphoid tissues. The CD95 (APO-1/Fas) ligand (CD95L) is induced in response to a variety of signals including TCR/CD3 stimulation or application of chemotherapeutic drugs. Here we report that an AP-1 site located in the 5′ untranslated region of the CD95L gene is required for TCR/CD3-mediated induction of the human CD95L promoter. Electrophoretic mobility shift assays using nuclear extracts of Jurkat T cells as well as TCR/CD3-restimulated primary human T cells demonstrated specific binding of AP-1, predominantly composed of c-Jun and FosB, to this sequence. Ectopic expression of transdominant negative Jun mutants strongly reduced CD95L promoter activity and activation-induced cell death (AICD), confirming the functional significance of FosB/c-Jun binding. Thus, our results demonstrate an important novel function for FosB dimerized with c-Jun in TCR/CD3-mediated AICD in human T cells.