| |  Online-Ressource |
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| Verfasst von: | Betzler, Alexander [VerfasserIn]  |
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| | Nanduri, Lahiri K. [VerfasserIn] |
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| | Hissa, Barbara [VerfasserIn] |
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| | Amtsfeld, Linda [VerfasserIn] |
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| | Muders, Michael Helmut [VerfasserIn] |
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| | Roy, Janine [VerfasserIn] |
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| | Jesinghaus, Moritz [VerfasserIn] |
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| | Steiger, Katja [VerfasserIn] |
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| | Weichert, Wilko [VerfasserIn] |
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| | Kloor, Matthias [VerfasserIn] |
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| | Klink, Barbara [VerfasserIn] |
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| | Schroeder, Michael [VerfasserIn] |
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| | Mazzone, Massimiliano [VerfasserIn] |
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| | Weitz, Jürgen [VerfasserIn] |
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| | Reißfelder, Christoph [VerfasserIn] |
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| | Rahbari, Nuh Nabi [VerfasserIn] |
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| | Schölch, Sebastian [VerfasserIn] |
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| Titel: | Differential effects of Trp53 alterations in murine colorectal cancer |
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| Verf.angabe: | Alexander M. Betzler, Lahiri K. Nanduri, Barbara Hissa, Linda Blickensdörfer, Michael H. Muders, Janine Roy, Moritz Jesinghaus, Katja Steiger, Wilko Weichert, Matthias Kloor, Barbara Klink, Michael Schroeder, Massimiliano Mazzone, Jürgen Weitz, Christoph Reissfelder, Nuh N. Rahbari and Sebastian Schölch |
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| E-Jahr: | 2021 |
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| Jahr: | 15 February 2021 |
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| Umfang: | 15 S. |
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| Fussnoten: | Gesehen am 15.04.2021 |
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| Titel Quelle: | Enthalten in: Cancers |
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| Ort Quelle: | Basel : MDPI, 2009 |
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| Jahr Quelle: | 2021 |
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| Band/Heft Quelle: | 13(2021), 4, Artikel-ID 808, Seite 1-15 |
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| ISSN Quelle: | 2072-6694 |
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| Abstract: | Background: Colorectal cancer (CRC) development is a multi-step process resulting in the accumulation of genetic alterations. Despite its high incidence, there are currently no mouse models that accurately recapitulate this process and mimic sporadic CRC. We aimed to develop and characterize a genetically engineered mouse model (GEMM) of Apc/Kras/Trp53 mutant CRC, the most frequent genetic subtype of CRC. Methods: Tumors were induced in mice with conditional mutations or knockouts in Apc, Kras, and Trp53 by a segmental adeno-cre viral infection, monitored via colonoscopy and characterized on multiple levels via immunohistochemistry and next-generation sequencing. Results: The model accurately recapitulates human colorectal carcinogenesis clinically, histologically and genetically. The Trp53 R172H hotspot mutation leads to significantly increased metastatic capacity. The effects of Trp53 alterations, as well as the response to treatment of this model, are similar to human CRC. Exome sequencing revealed spontaneous protein-modifying alterations in multiple CRC-related genes and oncogenic pathways, resulting in a genetic landscape resembling human CRC. Conclusions: This model realistically mimics human CRC in many aspects, allows new insights into the role of TP53 in CRC, enables highly predictive preclinical studies and demonstrates the value of GEMMs in current translational cancer research and drug development. |
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| DOI: | doi:10.3390/cancers13040808 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.3390/cancers13040808 |
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| | Volltext: https://www.mdpi.com/2072-6694/13/4/808 |
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| | DOI: https://doi.org/10.3390/cancers13040808 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | adenovirus |
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| | Apc |
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| | colorectal cancer |
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| | GEMM |
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| | genetically engineered mouse model |
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| | Kras |
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| | metastasis |
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| | preclinical studies |
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| | TP53 |
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| | Trp53 |
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| K10plus-PPN: | 1755007205 |
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| Verknüpfungen: | → Zeitschrift |
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Differential effects of Trp53 alterations in murine colorectal cancer / Betzler, Alexander [VerfasserIn]; 15 February 2021 (Online-Ressource)
