Status: Bibliographieeintrag
Standort: ---
Exemplare:
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| Online-Ressource |
Verfasst von: | Hentze, Hannes [VerfasserIn]  |
| Krammer, Peter H. [VerfasserIn]  |
Titel: | Glutathione dependence of caspase-8 activation at the death-inducing signaling complex |
Verf.angabe: | Hannes Hentze, Ingo Schmitz, Markus Latta, Andreas Krueger, Peter H. Krammer and Albrecht Wendel |
E-Jahr: | 2002 |
Jahr: | February 15, 2002 |
Umfang: | 8 S. |
Teil: | volume:277 |
| year:2002 |
| number:7 |
| pages:5588-5595 |
| extent:8 |
Fussnoten: | Gesehen am 15.04.2021 |
Titel Quelle: | Enthalten in: The journal of biological chemistry |
Ort Quelle: | Bethesda, Md. : Soc., 1905 |
Jahr Quelle: | 2002 |
Band/Heft Quelle: | 277(2002), 7, Seite 5588-5595 |
ISSN Quelle: | 1083-351X |
Abstract: | <p>Apoptosis triggered by the death receptor CD95 (APO-1 or Fas) is pivotal for the homeostasis of the immune system. We investigated differential effects of glutathione depletion on CD95-triggered apoptosis in T and B cell lines as well as the glutathione dependence of caspase-8 activation. In B lymphoblastoid SKW6.4 cells, CD95-mediated apoptosis was prevented upstream of caspase-8 activation and caspase-3-like activity after acute glutathione depletion by diethyl maleate or<i>cis</i>-chloro-dinitrobenzene. Immunoprecipitation of the death-inducing signaling complex (DISC) revealed that the DISC was still formed in the glutathione-depleted state. The first cleavage step of procaspase-8 activation at the DISC, however, was inhibited. Accordingly, under cell-free conditions, radiolabeled procaspase-8 was processed at the immunoprecipitated DISC only after the addition of exogenous dithiothreitol or reduced glutathione. We also observed suppression of CD95-mediated apoptosis in glutathione-depleted CEM and H9 cells. Notably, Jurkat cells still died upon CD95 engagement under this condition, displaying incomplete nuclear fragmentation and a partial switch to necrosis; this may be explained by reduced cytochrome<i>c</i>/dATP-mediated caspase activation observed in cytosol from glutathione-depleted Jurkat cytosol. Our data indicate that the activation of caspase-8 at the DISC and hence CD95-mediated apoptosis induction shows a cell-specific requirement for intracellular glutathione.</p> |
DOI: | doi:10.1074/jbc.M110766200 |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1074/jbc.M110766200 |
| Volltext: https://www.jbc.org/article/S0021-9258(19)82601-7/abstract |
| DOI: https://doi.org/10.1074/jbc.M110766200 |
Datenträger: | Online-Ressource |
Sprache: | eng |
K10plus-PPN: | 1755022042 |
Verknüpfungen: | → Zeitschrift |
Glutathione dependence of caspase-8 activation at the death-inducing signaling complex / Hentze, Hannes [VerfasserIn]; February 15, 2002 (Online-Ressource)
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