Lovastatin induces mitotic abnormalities in various cell lines

• Verfasst von: Lamprecht, Jan [VerfasserIn]
• Verfasst von: Paweletz, Neidhard [VerfasserIn]
• Titel: Lovastatin induces mitotic abnormalities in various cell lines
• Verf.angabe: Jan Lamprecht, Cezary Wójcik, Marek Jakóbisiak, Michael Stoehr, Dieter Schroeter and Neidhard Paweletz
• E-Jahr: 2013
• Jahr: 02 January 2013
• Jahr des Originals: 1999
• Umfang: 10 S.
• Teil: volume:23
• Teil: year:1999
• Teil: number:1
• Teil: pages:51-60
• Teil: extent:10
• Fussnoten: Elektronische Reproduktion der Druckausgabe ; Gesehen am 16.04.2021
• Titel Quelle: Enthalten in: Cell biology international
• Ort Quelle: Hoboken, NJ : Wiley-Blackwell, 1993
• Jahr Quelle: 1999
• Band/Heft Quelle: 23(1999), 1, Seite 51-60
• ISSN Quelle: 1095-8355
• DOI: doi:10.1006/cbir.1998.0322
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: aneuploidy
• Sach-SW: HMG-CoA reductase
• Sach-SW: lovastatin
• Sach-SW: mevalonate
• Sach-SW: mitosis
• K10plus-PPN: 1755138210

Abstract

We examined the effects of lovastatin, a common anti-atherosclerotic drug and a blocker of the cell cycle, on the process of mitosis. It is known that lovastatin induces an arrest or a retardation of the cell cycle in many cell types not only at the G1phase, but also at the G2/M transition. After 24-48h incubation of epithelial PtK2, T24, HeLa cells and fibroblastic L929 cells in the presence of 1.0-60.0μm lovastatin, diverse mitotic perturbations have been observed. The most noteworthy phenomena recorded were prometaphase retardation and chromosome lagging during metaphase and anaphase. After the recovery in lovastatin-free media, the cells continued mitosis without any disturbances. Mevalonic acid prevented the effects of lovastatin. We conclude that the effects were specific for lovastatin-induced inhibition of mevalonic acid synthesis. Immunofluorescence studies with anticentromeric antibodies suggested that one of the possible causes of the lovastatin-induced mitotic disorder could be an interference with the development and function of the centromeres.