Roles of CCAAT/enhancer-binding protein in transcriptional regulation of the human IL-5 gene

• Verfasst von: Li-Weber, Min [VerfasserIn]
• Verfasst von: Giaisi, Marco [VerfasserIn]
• Verfasst von: Krammer, Peter H. [VerfasserIn]
• Titel: Roles of CCAAT/enhancer-binding protein in transcriptional regulation of the human IL-5 gene
• Verf.angabe: Min Li-Weber, Marco Giaisi and Peter H. Krammer
• E-Jahr: 2001
• Jahr: 29 November 2001
• Umfang: 10 S.
• Teil: volume:31
• Teil: year:2001
• Teil: number:12
• Teil: pages:3694-3703
• Teil: extent:10
• Fussnoten: Gesehen am 21.04.2021
• Titel Quelle: Enthalten in: European journal of immunology
• Ort Quelle: Weinheim : Wiley-VCH, 1971
• Jahr Quelle: 2001
• Band/Heft Quelle: 31(2001), 12, Seite 3694-3703
• ISSN Quelle: 1521-4141
• DOI: doi:10.1002/1521-4141(200112)31:12<3694::AID-IMMU3694>3.0.CO;2-S
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: C/EBP
• Sach-SW: IL-5
• Sach-SW: T cell
• Sach-SW: Tax
• K10plus-PPN: 1755487517

Abstract

Abstract Interleukin-5 (IL-5) plays a critical role in the pathogenesis of eosinophil-associated allergic disorders, such as asthma. IL-5 may also play a major role in the development of eosinophilia-associated lymphoproliferative disorders caused by human T lymphotropic virus type?I (HTLV-I). In this study, we have investigated the control mechanisms for IL-5 production and found that ectopic expression of NF-IL6 (C/EBP?) increases endogenous IL-5 mRNA expression. The IL-5 promoter contains four C/EBP consensus sequences. We show here that one of the C/EBP site at ??235 promoter region binds to NF-IL6 protein with high affinity and interacts with NF-IL6 and NF-IL6? (C/EBPδ) in Jurkat T?cells. Mutations within the C/EBP sequence reduced the promoter activity in response to T?cell activation by more than 50?%. In addition, we show that in vivo inducible expression of Tax protein in Jurkat T?cells stably transfected with Tax further increased ionomycin plus phorbol ester stimulated IL-5 promoter activity. The effect of Tax on IL-5 promoter activity was abolished when the C/EBP site was mutated. Thus, the C/EBP site may be also involved in HTLV-I Tax-mediated up-regulation of IL-5 gene expression. Our data suggest that C/EBP proteins may regulate IL-5 gene expression in response to different stimulation signals.