Stability of cannabinoids in hair samples exposed to sunlight

• Verfasst von: Skopp, Gisela [VerfasserIn]
• Verfasst von: Pötsch-Schneider, Lucia Edeltraud [VerfasserIn]
• Verfasst von: Mauden, Jörg Martin [VerfasserIn]
• Titel: Stability of cannabinoids in hair samples exposed to sunlight
• Verf.angabe: Gisela Skopp, Lucia Pötsch, and Martin Mauden
• E-Jahr: 2000
• Jahr: 01 November 2000
• Umfang: 3 S.
• Teil: volume:46
• Teil: year:2000
• Teil: number:11
• Teil: pages:1846-1848
• Teil: extent:3
• Fussnoten: Gesehen am 21.04.2021
• Titel Quelle: Enthalten in: Clinical chemistry
• Ort Quelle: Washington, DC : American Association for Clinical Chemistry, 1955
• Jahr Quelle: 2000
• Band/Heft Quelle: 46(2000), 11, Seite 1846-1848
• ISSN Quelle: 1530-8561
• DOI: doi:10.1093/clinchem/46.11.1846
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1755600771

Abstract

The cannabinoid content in the hair samples was determined by gas chromatography-mass spectrometry. Each strand was pulverized in a ball mill, and ∼50 mg of the hair powder was weighed; 25 ng of THC-d3 (Radian International) was added as internal standard, and the sample was further processed according to Cirimele et al. (12). A 1-μL aliquot of the extract was injected into a gas chromatography-mass spectrometry system [HP 6890 gas chromatograph and HP 6890 mass spectrometer, both from Hewlett Packard; column, CP-Sil 5, 12.5 m × 0.53 mm (i.d.), from Chrompack]. Detection was by ionization in the electron impact mode (70 eV), with the scan mode set at single-ion monitoring (THC, m/z 299, 314, 271; THC-d3, m/z 302, 317, 274; CBN, m/z 295, 310, 238; CBD, m/z 231, 246, 314). Measurements were taken twice from each strand. The THC concentrations determined from a particular sample differed by at least 15% and are given as mean values (n = 4; Table 1 ). Deuterated standards for CBN and CDB are not yet commercially available; hence, these compounds were not quantified, and triplicate measurements of six drug-free hair samples were used to estimate their particular detection limits (mean + 3 SD). The limit of detection was 0.05 ng/mg of hair for all analytes.