| Online-Ressource |
Verfasst von: | Müller-Decker, Karin [VerfasserIn]  |
| Krieg, Peter [VerfasserIn]  |
| Bayerl, Christiane [VerfasserIn]  |
| Marks, Friedrich [VerfasserIn]  |
| Fürstenberger, Gerhard [VerfasserIn]  |
Titel: | Prostaglandin-H-synthase isozyme expression in normal and neoplastic human skin |
Verf.angabe: | Karin Müller‐Decker, Günther Reinerth, Peter Krieg, Regina Zimmermann, Helmut Heise, Christiane Bayerl, Friedrich Marks and Gerhard Fürstenberger |
E-Jahr: | 1999 |
Jahr: | 10 November 1999 |
Umfang: | 9 S. |
Fussnoten: | Gesehen am 21.04.2021 |
Titel Quelle: | Enthalten in: International journal of cancer |
Ort Quelle: | Bognor Regis : Wiley-Liss, 1966 |
Jahr Quelle: | 1999 |
Band/Heft Quelle: | 82(1999), 5, Seite 648-656 |
ISSN Quelle: | 1097-0215 |
Abstract: | Expression of prostaglandin-H-synthase (PGHS) isozymes was analyzed in 50 biopsies of normal human skin and of pre-malignant and malignant skin lesions, by means of quantitative RT-PCR, immunoprecipitation and Western blotting, as well as immunohistochemistry. Normal skin constitutively expressed PGHS-1 in all cell layers of the epidermis, in endothelial cells of small blood vessels and in sweat-gland epithelium. PGHS-2 expression was very low and restricted to a few keratinocytes of the interfollicular and follicular epidermis. Steady-state concentrations of PGHS-1 and PGHS-2 mRNA were similar in normal skin and in basal-cell carcinomas, but PGHS-1 mRNA was reduced and PGHS-2 mRNA was elevated in actinic keratoses, squamous-cell carcinomas and keratoacanthomas. PGHS-1 protein was detected in all tumor biopsies, being occasionally increased in basal-cell carcinomas. High amounts of PGHS-2 protein were found in actinic keratoses, squamous-cell carcinomas and keratoacanthomas, but not in basal-cell carcinomas. Four malignant melanomas included in this study contained PGHS-1 but no PGHS-2 protein. Immunohistochemical analysis of the biopsies identified keratinocytes, in addition to cells of inflammatory infiltrates and of dendritic morphology, as the major PGHS-expressing cell types. PGHS-2-specific signals were spread throughout the epidermal part of actinic keratoses and squamous-cell carcinomas. These data suggest that constitutive up-regulation of PGHS-2 expression is a consistent pre-malignant event in squamous-cell cancer development in man, as it is in animal models of skin carcinogenesis. Thus, pre-cancerous lesions such as actinic keratoses present a likely target for chemoprevention of skin cancer by selective PGHS-2 inhibitors. |
DOI: | doi:10.1002/(SICI)1097-0215(19990827)82:5<648::AID-IJC6>3.0.CO;2-D |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/https://doi.org/10.1002/(SICI)1097-0215(19990827)82:5<648::AID-IJC6>3.0.CO;2-D |
| Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1002/%28SICI%291097-0215%2819990827%2982%3A5%3C648%3A%3AAID-IJC6%3E3.0.CO%3B2 ... |
| DOI: https://doi.org/10.1002/(SICI)1097-0215(19990827)82:5<648::AID-IJC6>3.0.CO;2-D |
Datenträger: | Online-Ressource |
Sprache: | eng |
K10plus-PPN: | 1755610181 |
Verknüpfungen: | → Zeitschrift |
Prostaglandin-H-synthase isozyme expression in normal and neoplastic human skin / Müller-Decker, Karin [VerfasserIn]; 10 November 1999 (Online-Ressource)