Partition coefficient, blood to plasma ratio, protein binding and short-term stability of 11-nor-Δ9-carboxy tetrahydrocannabinol glucuronide

• Verfasst von: Skopp, Gisela [VerfasserIn]
• Verfasst von: Pötsch-Schneider, Lucia Edeltraud [VerfasserIn]
• Verfasst von: Mauden, Jörg Martin [VerfasserIn]
• Verfasst von: Richter, Barbara [VerfasserIn]
• Titel: Partition coefficient, blood to plasma ratio, protein binding and short-term stability of 11-nor-Δ9-carboxy tetrahydrocannabinol glucuronide
• Verf.angabe: Gisela Skopp, Lucia Pötsch, Martin Mauden, Barbara Richter
• E-Jahr: 2002
• Jahr: 16 February 2002
• Umfang: 7 S.
• Teil: volume:126
• Teil: year:2002
• Teil: number:1
• Teil: pages:17-23
• Teil: extent:7
• Fussnoten: Gesehen am 21.04.2021
• Titel Quelle: Enthalten in: Forensic science international
• Ort Quelle: Amsterdam [u.a.] : Elsevier Science, 1978
• Jahr Quelle: 2002
• Band/Heft Quelle: 126(2002), 1, Seite 17-23
• ISSN Quelle: 1872-6283
• DOI: doi:10.1016/S0379-0738(02)00023-3
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: 11-Nor-Δ-carboxy tetrahydrocannabinol glucuronide
• Sach-SW: Blood to plasma ratio
• Sach-SW: Partition coefficient
• Sach-SW: Protein binding
• Sach-SW: Short-term stability
• K10plus-PPN: 1755639465

Abstract

11-Nor-Δ9-carboxy tetrahydrocannabinol glucuronide (THCCOOglu) is a major metabolite of tetrahydrocannabinol in blood. Despite its mass spectrometric identification already in 1980, further physicochemical data of THCCOOglu have not been established. Therefore, the octanol/buffer partition coefficient P and the blood to plasma ratio b/p for THCCOOglu concentrations of 100 and 500ng/ml were investigated. Protein binding of the glucuronide was established from spiked albumin solutions at a level of 250ng/ml as well as from authentic samples. The data were compared to those of 11-nor-Δ9-carboxy tetrahydrocannabinol (THCCOOH). In addition, the short-term stability of THCCOOglu in plasma at different storage temperatures was studied. Analysis was performed by LC/MS/MS. The glucuronide partition coefficient P (mean: 17.4 and 18.0 for 100 and 500ng/ml, respectively) was unexpectedly lipophilic at pH 7.4. Its blood to plasma ratios averaged 0.62 and 0.68 at 100 and 500ng/ml, respectively. THCCOOglu was highly reversibly bound to albumin (mean: 97%), and the mean fraction bound did not differ from that determined from authentic samples. THCCOOglu degraded even at a storage temperature of 4°C and THCCOOH was identified as a major decomposition product.