cDNA cloning, genomic structure, and chromosomal localization of a novel murine epidermis-type lipoxygenase

• Verfasst von: Kinzig, Andreas [VerfasserIn]
• Verfasst von: Fürstenberger, Gerhard [VerfasserIn]
• Verfasst von: Marks, Friedrich [VerfasserIn]
• Verfasst von: Krieg, Peter [VerfasserIn]
• Titel: cDNA cloning, genomic structure, and chromosomal localization of a novel murine epidermis-type lipoxygenase
• Verf.angabe: Andreas Kinzig, Markus Heidt, Gerhard Fürstenberger, Friedrich Marks, and Peter Krieg
• E-Jahr: 2002
• Jahr: 25 May 2002
• Jahr des Originals: 1999
• Umfang: 7 S.
• Teil: volume:58
• Teil: year:1999
• Teil: number:2
• Teil: pages:158-164
• Teil: extent:7
• Fussnoten: Elektronische Reproduktion der Druckausgabe ; Gesehen am 22.04.2021
• Titel Quelle: Enthalten in: Genomics
• Ort Quelle: San Diego, Calif. : Academic Press, 1987
• Jahr Quelle: 1999
• Band/Heft Quelle: 58(1999), 2, Seite 158-164
• ISSN Quelle: 1089-8646
• DOI: doi:10.1006/geno.1999.5816
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1755732783

Abstract

Using a combination of degenerate PCR technique and conventional screening procedures, we isolated a cDNA encoding a novel lipoxygenase, termed epidermis-type lipoxygenase-3 (e-LOX-3, gene symbol Aloxe3), from mouse skin. Aloxe3 mRNA is expressed in the stratified epithelia of skin, tongue, and forestomach. The cDNA encodes a protein of 711 amino acids with a calculated molecular mass of 80.6 kDa. The amino acid sequence shows approximately 54% identity to the recently identified 12(R)-lipoxygenase. Sequence comparison revealed a segment of 41 amino acid residues localized near the boundary between the N- and the C-terminal domain sequences of the molecule, a structural feature that is also characteristic of 12(R)-lipoxygenase, suggesting that these two epidermis-derived lipoxygenases may be members of a novel structural class of mammalian lipoxygenases. The novel lipoxygenase gene is divided into 15 exons and 14 introns, spanning 22.3 kb of genomic DNA. By interspecific backcross analysis, the novel gene was localized to the central region of mouse chromosome 11.