The structure of the γ-TuRC

• Verfasst von: Župa, Erik [VerfasserIn]
• Verfasst von: Liu, Peng [VerfasserIn]
• Verfasst von: Würtz, Martin [VerfasserIn]
• Verfasst von: Schiebel, Elmar [VerfasserIn]
• Verfasst von: Pfeffer, Stefan [VerfasserIn]
• Titel: The structure of the γ-TuRC
• Titelzusatz: a 25-years-old molecular puzzle
• Verf.angabe: Erik Zupa, Peng Liu, Martin Würtz, Elmar Schiebel and Stefan Pfeffer
• Jahr: 2021
• Umfang: 7 S.
• Fussnoten: Available online 29 September 2020 ; Gesehen am 06.08.2021
• Titel Quelle: Enthalten in: Current opinion in structural biology
• Ort Quelle: Amsterdam [u.a.] : Elsevier, 1991
• Jahr Quelle: 2021
• Band/Heft Quelle: 66(2021) vom: Feb., Seite 15-21
• DOI: doi:10.1016/j.sbi.2020.08.008
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1755787545

Abstract

The nucleation of microtubules from αβ-tubulin dimers is an essential cellular process dependent on γ-tubulin complexes. Mechanistic understanding of the nucleation reaction was hampered by the lack of γ-tubulin complex structures at sufficiently high resolution. The recent technical developments in cryo-electron microscopy have allowed resolving the vertebrate γ-tubulin ring complex (γ-TuRC) structure at near-atomic resolution. These studies clarified the arrangement and stoichiometry of gamma-tubulin complex proteins in the γ-TuRC, characterized the surprisingly versatile integration of the small proteins MZT1/2 into the complex, and identified actin as an integral component of the γ-TuRC. In this review, we summarize the structural insights into the molecular architecture, the assembly pathway, and the regulation of the microtubule nucleation reaction.