Superhelical DNA studied by solution scattering and computer models

• Verfasst von: Langowski, Jörg [VerfasserIn]
• Verfasst von: Klenin, Konstantin [VerfasserIn]
• Verfasst von: Tóth, Katalin [VerfasserIn]
• Titel: Superhelical DNA studied by solution scattering and computer models
• Verf.angabe: Jörg Langowski, Markus Hammermann, Konstantin Klenin, Roland May & Katalin Tóth
• E-Jahr: 1999
• Jahr: June 1999
• Umfang: 7 S.
• Fussnoten: Gesehen am 23.04.2021
• Titel Quelle: Enthalten in: Genetica
• Ort Quelle: Dordrecht [u.a.] : Springer Science + Business Media B.V, 1919
• Jahr Quelle: 1999
• Band/Heft Quelle: 106(1999), 1, Seite 49-55
• ISSN Quelle: 1573-6857
• DOI: doi:10.1023/A:1003720610089
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1755829396

Abstract

We present here recent results on the structure of superhelical DNA and its changes with salt concentration between 0.01 and 1.5 M NaCl. Scattering curves of two different superhelical DNAs were determined by static light scattering. The measured radii of gyration do not change significantly with salt concentration. Small-angle neutron scattering, together with calculations from a Monte Carlo model, allows to determine the superhelix diameter. Measured and simulated scattering curves agreed almost quantitatively. Experimentally we find that the diameter decreases from 16.0±0.9 nm at 10 mM to 9.0±0.7 nm at 100 mM NaCl. The superhelix diameter from the simulated conformations decreased from 18.0±1.5 nm at 10 mM to 9.4±1.5 nm at 100 mM NaCl. At higher salt concentrations up to 1.5 M NaCl, the diameter stays constant at 9 nm.