Vacuolating cytotoxin of helicobacter pylori induces apoptosis in the human gastric epithelial cell line AGS

• Verfasst von: Kuck, Dirk [VerfasserIn]
• Verfasst von: Krammer, Peter H. [VerfasserIn]
• Verfasst von: Stremmel, Wolfgang [VerfasserIn]
• Verfasst von: Rudi, Jochen [VerfasserIn]
• Titel: Vacuolating cytotoxin of helicobacter pylori induces apoptosis in the human gastric epithelial cell line AGS
• Verf.angabe: Dirk Kuck, Bernhard Kolmerer, Christof Iking-Konert, Peter H. Krammer, Wolfgang Stremmel, and Jochen Rudi
• E-Jahr: 2001
• Jahr: August 1, 2001
• Umfang: 8 S.
• Teil: volume:69
• Teil: year:2001
• Teil: number:8
• Teil: pages:5080-5087
• Teil: extent:8
• Fussnoten: Gesehen am 23.04.2021
• Titel Quelle: Enthalten in: Infection and immunity
• Ort Quelle: Washington, DC : Soc., 1970
• Jahr Quelle: 2001
• Band/Heft Quelle: 69(2001), 8, Seite 5080-5087
• ISSN Quelle: 1098-5522
• DOI: doi:10.1128/IAI.69.8.5080-5087.2001
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1755845103

Abstract

Helicobacter pylori induces cell death by apoptosis. However, the apoptosis-inducing factor is still unknown. The virulence factor vacuolating cytotoxin A (VacA) is a potential candidate, and thus its role in apoptosis induction was investigated in the human gastric epithelial cell line AGS. The supernatant from thevacA wild-type strain P12 was able to induce apoptotic cell death, whereas the supernatant from its isogenic mutant strain P14 could not. That VacA was indeed the apoptosis-inducing factor was demonstrated further by substantial reduction of apoptosis upon treatment of AGS cells with a supernatant specifically depleted of native VacA. Furthermore, a recombinant VacA produced inEscherichia coli was also able to induce apoptosis in AGS cells but failed to induce cellular vacuolation. These findings demonstrate that the vacuolating cytototoxin of H. pyloriis a bacterial factor capable of inducing apoptosis in gastric epithelial cells.