Status: Bibliographieeintrag
Standort: ---
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| Online-Ressource |
Verfasst von: | Krueger, Andreas [VerfasserIn]  |
| Krammer, Peter H. [VerfasserIn]  |
Titel: | Cellular FLICE-inhibitory protein splice variants inhibit different steps of caspase-8 activation at the CD95 death-inducing signaling complex* |
Verf.angabe: | Andreas Krueger, Ingo Schmitz, Sven Baumann, Peter H. Krammer, and Sabine Kirchhoff |
Jahr: | 2001 |
Umfang: | 8 S. |
Teil: | volume:276 |
| year:2001 |
| number:23 |
| pages:20633-20640 |
| extent:8 |
Fussnoten: | Elektronische Reproduktion der Druck-Ausgabe ; Available online 4 January 2021 ; Gesehen am 26.04.2021 |
Titel Quelle: | Enthalten in: The journal of biological chemistry |
Ort Quelle: | Bethesda, Md. : Soc., 1905 |
Jahr Quelle: | 2001 |
Band/Heft Quelle: | 276(2001), 23, Seite 20633-20640 |
ISSN Quelle: | 1083-351X |
Abstract: | Upon stimulation, CD95 (APO-1/Fas) recruits the adapter molecule FADD/MORT1, procaspase-8, and the cellular FLICE-inhibitory proteins (c-FLIP) into the death-inducing signaling complex (DISC). According to the induced proximity model, procaspase-8 is activated in the DISC in an autoproteolytic manner by two subsequent cleavage steps. c-FLIP proteins exist as a long (c-FLIPL) and a short (c-FLIPS) splice variant, both of them capable of protecting cells from death receptor-mediated apoptosis. In stably transfected BJAB cells, both c-FLIPS and c-FLIPL block procaspase-8 activation at the DISC. However, cleavage is blocked at different steps. c-FLIPL allows the first cleavage step of procaspase-8, leading to the generation of the p10 subunit. In contrast, c-FLIPS completely inhibits cleavage of procaspase-8. Interestingly, p43-c-FLIPL lacking the p12 subunit also prevents cleavage of procaspase-8. In contrast, a nonprocessable mutant of c-FLIPL allows the first cleavage of procaspase-8. In conclusion, both c-FLIP proteins prevent caspase-8 activation at different levels of procaspase-8 processing at the DISC. Our results indicate that c-FLIPL induces a conformation of procaspase-8 that allows partial but not complete proteolytical processing, whereas in contrast c-FLIPS even prevents partial procaspase-8 activation at the DISC. |
DOI: | doi:10.1074/jbc.M101780200 |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1074/jbc.M101780200 |
| Volltext: https://www.sciencedirect.com/science/article/pii/S002192581940519X |
| DOI: https://doi.org/10.1074/jbc.M101780200 |
Datenträger: | Online-Ressource |
Sprache: | eng |
K10plus-PPN: | 1755925409 |
Verknüpfungen: | → Zeitschrift |
Cellular FLICE-inhibitory protein splice variants inhibit different steps of caspase-8 activation at the CD95 death-inducing signaling complex* / Krueger, Andreas [VerfasserIn]; 2001 (Online-Ressource)
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