Status: Bibliographieeintrag
Standort: ---
Exemplare:
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| Online-Ressource |
Verfasst von: | Kirchhoff, Sabine [VerfasserIn]  |
| Krammer, Peter H. [VerfasserIn]  |
Titel: | TCR-mediated up-regulation of c-FLIPshort correlates with resistance toward CD95-mediated apoptosis by blocking death-inducing signaling complex activity |
Verf.angabe: | Sabine Kirchhoff, Wolfgang W. Müller, Andreas Krueger, Ingo Schmitz, and Peter H. Krammer |
E-Jahr: | 2000 |
Jahr: | December 1, 2000 |
Umfang: | 8 S. |
Teil: | volume:165 |
| year:2000 |
| number:11 |
| pages:6293-6300 |
| extent:8 |
Fussnoten: | Gesehen am 26.04.2021 ; Im Titel ist der Ausdruck "short" tiefgestellt |
Titel Quelle: | Enthalten in: The journal of immunology |
Ort Quelle: | Bethesda, Md. : Soc., 1916 |
Jahr Quelle: | 2000 |
Band/Heft Quelle: | 165(2000), 11, Seite 6293-6300 |
ISSN Quelle: | 1550-6606 |
Abstract: | To investigate apoptosis resistance upon restimulation in human peripheral blood T lymphocytes, we used the following in vitro model. This model represents the main features of T cell reactivity: freshly isolated PHA-activated T cells cultured in IL-2 for a prolonged period of time develop a CD95 (APO-1/Fas) apoptosis-sensitive phenotype. These T cells represent activation-induced cell death-sensitive T cells during the down phase of an immune response. A fraction of apoptosis-sensitive activated T cells becomes apoptosis resistant upon TCR/CD3 restimulation. CD95 apoptosis sensitivity requires formation of a functional receptor associated death-inducing signaling complex (DISC), i.e., a protein complex of CD95 receptors, the adaptor Fas-associated death domain protein (FADD)/MORT1 and caspase-8 (FADD-like IL-1β-converting enzyme (FLICE), MACH, Mch5). We identified activation of procaspase-8 at the DISC as the main target for the protective activity of TCR/CD3 restimulation. We found that procaspase-8 cleavage is reduced in T cells after TCR/CD3 restimulation. In addition, we detected up-regulation of c-FLIPS (the short splice variant of the cellular FLICE inhibitory protein) and strongly enhanced recruitment of c-FLIPS into the DISC. These data suggest that the recruitment of c-FLIPS into the DISC results in reduced DISC and caspase-8 activity. |
DOI: | doi:10.4049/jimmunol.165.11.6293 |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.4049/jimmunol.165.11.6293 |
| Volltext: https://www-jimmunol-org.ubproxy.ub.uni-heidelberg.de/content/165/11/6293 |
| DOI: https://doi.org/10.4049/jimmunol.165.11.6293 |
Datenträger: | Online-Ressource |
Sprache: | eng |
K10plus-PPN: | 1755927908 |
Verknüpfungen: | → Zeitschrift |
TCR-mediated up-regulation of c-FLIPshort correlates with resistance toward CD95-mediated apoptosis by blocking death-inducing signaling complex activity / Kirchhoff, Sabine [VerfasserIn]; December 1, 2000 (Online-Ressource)
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