Up-regulation of c-FLIPshort and reduction of activation-induced cell death in CD28-co-stimulated human T cells

• Verfasst von: Kirchhoff, Sabine [VerfasserIn]
• Verfasst von: Li-Weber, Min [VerfasserIn]
• Verfasst von: Krammer, Peter H. [VerfasserIn]
• Titel: Up-regulation of c-FLIPshort and reduction of activation-induced cell death in CD28-co-stimulated human T cells
• Verf.angabe: Sabine Kirchhoff, Wolfgang W. Müller, Min Li‐Weber and Peter H. Krammer
• E-Jahr: 2000
• Jahr: 29 November 2000
• Umfang: 10 S.
• Teil: volume:30
• Teil: year:2000
• Teil: number:10
• Teil: pages:2765-2774
• Teil: extent:10
• Fussnoten: Gesehen am 26.04.2021 ; Im Titel ist der Ausdruck "short" tiefgestellt
• Titel Quelle: Enthalten in: European journal of immunology
• Ort Quelle: Weinheim : Wiley-VCH, 1971
• Jahr Quelle: 2000
• Band/Heft Quelle: 30(2000), 10, Seite 2765-2774
• ISSN Quelle: 1521-4141
• DOI: doi:10.1002/1521-4141(200010)30:10<2765::AID-IMMU2765>3.0.CO;2-W
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Activation-induced cell death
• Sach-SW: CD95 (APO-1/Fas)
• Sach-SW: Co-stimulation
• Sach-SW: FLICE-inhibitory protein
• Sach-SW: T cell
• K10plus-PPN: 1755936206

Abstract

Efficient activation of antigen-specific T cells requires co-stimulatory signals provided e.g. by CD28. Re-exposure to antigen and CD28 co-stimulation reduces activation-induced cell death (AICD) and increases the number of T cells performing effector functions. AICD is mediated predominantly by CD95 (APO-1/Fas) and its cognate ligand (CD95L). In an in vitro model system, using human peripheral activated T cells, we demonstrate here that co-stimulation prevents CD95L expression. Moreover, we show that co-stimulation reduces the activity of the CD95 death-inducing signaling complex and procaspase-8 activation. In parallel, co-stimulation strongly increases expression of the short form of the FLICE-inhibitory protein c-FLIPshort and of Bcl-xL. These data provide important new insight into the molecular mechanisms of apoptosis resistance in co-stimulated T cells.