Status: Bibliographieeintrag
Standort: ---
Exemplare:
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| Online-Ressource |
Verfasst von: | Li-Weber, Min [VerfasserIn]  |
| Krammer, Peter H. [VerfasserIn]  |
Titel: | T cell activation-induced and HIV Tat-enhanced CD95(APO-1 / Fas) ligand transcription involves NF-κB |
Verf.angabe: | Min Li‐Weber, Oskar Laur, Kirsten Dern and Peter H. Krammer |
E-Jahr: | 2000 |
Jahr: | 28 August 2000 |
Umfang: | 10 S. |
Teil: | volume:30 |
| year:2000 |
| number:2 |
| pages:661-670 |
| extent:10 |
Fussnoten: | Gesehen am 26.04.2021 |
Titel Quelle: | Enthalten in: European journal of immunology |
Ort Quelle: | Weinheim : Wiley-VCH, 1971 |
Jahr Quelle: | 2000 |
Band/Heft Quelle: | 30(2000), 2, Seite 661-670 |
ISSN Quelle: | 1521-4141 |
Abstract: | CD95(APO-1 / Fas) ligand (CD95L) gene expression is critically involved in activation-induced T cell apoptosis. We and other have previously shown that HIV-1 Tat which is essential for efficient HIV gene expression sensitizes CD95-mediated apoptosis and up-regulates CD95L expression in T cells. In the present study we have investigated the regulatory mechanism for CD95L expression. Two NF-κB binding sites are localized at − 537 to − 521 and − 57 to − 47 (relative to the transcription start site) of the human CD95L promoter. We show that both elements bind to NF-κB and SP-1 transcription factors and NF-κB is involved in transactivation of the CD95L promoter upon T cell activation. Mutations at each NF-κB site by two base pair substitutions resulted in 30 - 70 % reduction of the promoter activity. The effect of Tat on the human CD95L promoter activity was mapped to the same sites. Mutation of each NF-κB site also impaired the effect of Tat on CD95L promotor activity. We also show that ectopic expression of Tat protein in Jurkat T cells greatly increases NF-κB binding to its target DNA. Our studies provide evidence that Tat-enhanced CD95L expression is regulated at least in part by the NF-κB sites of the promoter. |
DOI: | doi:10.1002/1521-4141(200002)30:2<661::AID-IMMU661>3.0.CO;2-L |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/https://doi.org/10.1002/1521-4141(200002)30:2<661::AID-IMMU661>3.0.CO;2-L |
| Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1002/1521-4141%28200002%2930%3A2%3C661%3A%3AAID-IMMU661%3E3.0.CO%3B2-L |
| DOI: https://doi.org/10.1002/1521-4141(200002)30:2<661::AID-IMMU661>3.0.CO;2-L |
Datenträger: | Online-Ressource |
Sprache: | eng |
Sach-SW: | AIDS |
| Apoptosis |
| CD95(APO-1 / Fas) ligand promoter |
| T cell |
K10plus-PPN: | 1755938101 |
Verknüpfungen: | → Zeitschrift |
T cell activation-induced and HIV Tat-enhanced CD95(APO-1 / Fas) ligand transcription involves NF-κB / Li-Weber, Min [VerfasserIn]; 28 August 2000 (Online-Ressource)
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