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Verfasst von:Li-Weber, Min [VerfasserIn]   i
 Krammer, Peter H. [VerfasserIn]   i
Titel:T cell activation-induced and HIV Tat-enhanced CD95(APO-1 / Fas) ligand transcription involves NF-κB
Verf.angabe:Min Li‐Weber, Oskar Laur, Kirsten Dern and Peter H. Krammer
E-Jahr:2000
Jahr:28 August 2000
Umfang:10 S.
Teil:volume:30
 year:2000
 number:2
 pages:661-670
 extent:10
Fussnoten:Gesehen am 26.04.2021
Titel Quelle:Enthalten in: European journal of immunology
Ort Quelle:Weinheim : Wiley-VCH, 1971
Jahr Quelle:2000
Band/Heft Quelle:30(2000), 2, Seite 661-670
ISSN Quelle:1521-4141
Abstract:CD95(APO-1 / Fas) ligand (CD95L) gene expression is critically involved in activation-induced T cell apoptosis. We and other have previously shown that HIV-1 Tat which is essential for efficient HIV gene expression sensitizes CD95-mediated apoptosis and up-regulates CD95L expression in T cells. In the present study we have investigated the regulatory mechanism for CD95L expression. Two NF-κB binding sites are localized at − 537 to − 521 and − 57 to − 47 (relative to the transcription start site) of the human CD95L promoter. We show that both elements bind to NF-κB and SP-1 transcription factors and NF-κB is involved in transactivation of the CD95L promoter upon T cell activation. Mutations at each NF-κB site by two base pair substitutions resulted in 30 - 70 % reduction of the promoter activity. The effect of Tat on the human CD95L promoter activity was mapped to the same sites. Mutation of each NF-κB site also impaired the effect of Tat on CD95L promotor activity. We also show that ectopic expression of Tat protein in Jurkat T cells greatly increases NF-κB binding to its target DNA. Our studies provide evidence that Tat-enhanced CD95L expression is regulated at least in part by the NF-κB sites of the promoter.
DOI:doi:10.1002/1521-4141(200002)30:2<661::AID-IMMU661>3.0.CO;2-L
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext ; Verlag: https://doi.org/https://doi.org/10.1002/1521-4141(200002)30:2<661::AID-IMMU661>3.0.CO;2-L
 Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1002/1521-4141%28200002%2930%3A2%3C661%3A%3AAID-IMMU661%3E3.0.CO%3B2-L
 DOI: https://doi.org/10.1002/1521-4141(200002)30:2<661::AID-IMMU661>3.0.CO;2-L
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:AIDS
 Apoptosis
 CD95(APO-1 / Fas) ligand promoter
 T cell
K10plus-PPN:1755938101
Verknüpfungen:→ Zeitschrift

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