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Verfasst von:Stegmann, Heiko [VerfasserIn]   i
 Wepf, Roger [VerfasserIn]   i
 Schröder, Rasmus R. [VerfasserIn]   i
 Fink, Rainer [VerfasserIn]   i
Titel:Quantification of total calcium in terminal cisternae of skinned muscle fibers by imaging electron energy-loss spectroscopy
Verf.angabe:Heiko Stegmann, Roger Wepf, Rasmus R. Schröder and Rainer H.A. Fink
E-Jahr:1999
Jahr:[1999]
Umfang:11 S.
Illustrationen:Illustrationen
Teil:volume:20
 year:1999
 number:5
 pages:505-515
 extent:11
Fussnoten:Gesehen am 27.04.2021
Titel Quelle:Enthalten in: Journal of muscle research and cell motility
Ort Quelle:Dordrecht [u.a.] : Springer Science + Business Media B.V, 1980
Jahr Quelle:1999
Band/Heft Quelle:20(1999), 5, Seite 505-515
ISSN Quelle:1573-2657
Abstract:Skinned muscle fibers are ideal model preparations for the investigation of Ca2+-regulatory mechanisms. Their internal ionic milieu can be easily controlled and distinct physiological states are well defined. We have measured the total Ca content in the terminal cisternae of such preparations using imaging electron energy-loss spectroscopy (Image-EELS) as a new approach for quantification of sub-cellular element distributions. Murine muscle fibers submitted to a standardized calcium-loading procedure were cryo-fixed with a combined solution exchanger/plunge freezing device. Energy-filtered image series were recorded from ultrathin freeze-dried cryosections of samples immobilized in either relaxed or caffeine-contracted state. From these image series, electron energy-loss spectra were extracted by digital image-processing and quantitatively processed by multiple-least-squares-fitting with reference spectra. The calculated fit coefficients were converted to Ca-concentrations by a calibration obtained from Ca-standards. Total Ca-contents in the terminal cisternae of skinned skeletal muscle fibers decreased upon caffeine-induced Ca-release from 123 ± 159 (±11) to 73 ± 102 (±8) mmol/kg d.w. (weighted mean ± SD (±SEM)).
DOI:doi:10.1023/A:1005522912044
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext: https://doi.org/10.1023/A:1005522912044
 DOI: https://doi.org/10.1023/A:1005522912044
Datenträger:Online-Ressource
Sprache:eng
K10plus-PPN:175599429X
Verknüpfungen:→ Zeitschrift

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