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| Verfasst von: | Dobersch, Stephanie [VerfasserIn]  |
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| | Rubio, Karla [VerfasserIn] |
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| | Singh, Indrabahadur [VerfasserIn] |
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| | Günther, Stefan [VerfasserIn] |
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| | Graumann, Johannes [VerfasserIn] |
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| | Cordero, Julio [VerfasserIn] |
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| | Castillo-Negrete, Rafael [VerfasserIn] |
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| | Huynh, Minh Bao [VerfasserIn] |
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| | Mehta, Aditi [VerfasserIn] |
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| | Braubach, Peter [VerfasserIn] |
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| | Cabrera-Fuentes, Hector [VerfasserIn] |
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| | Bernhagen, Jürgen [VerfasserIn] |
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| | Chao, Cho-Ming [VerfasserIn] |
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| | Bellusci, Saverio [VerfasserIn] |
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| | Günther, Andreas [VerfasserIn] |
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| | Preissner, Klaus T. [VerfasserIn] |
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| | Kugel, Sita [VerfasserIn] |
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| | Dobreva, Gergana [VerfasserIn] |
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| | Wygrecka, Malgorzata [VerfasserIn] |
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| | Braun, Thomas [VerfasserIn] |
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| | Papy-Garcia, Dulce [VerfasserIn] |
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| | Barreto, Guillermo [VerfasserIn] |
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| Titel: | Positioning of nucleosomes containing γ-H2AX precedes active DNA demethylation and transcription initiation |
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| Verf.angabe: | Stephanie Dobersch, Karla Rubio, Indrabahadur Singh, Stefan Günther, Johannes Graumann, Julio Cordero, Rafael Castillo-Negrete, Minh Bao Huynh, Aditi Mehta, Peter Braubach, Hector Cabrera-Fuentes, Jürgen Bernhagen, Cho-Ming Chao, Saverio Bellusci, Andreas Günther, Klaus T. Preissner, Sita Kugel, Gergana Dobreva, Malgorzata Wygrecka, Thomas Braun, Dulce Papy-Garcia and Guillermo Barreto |
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| E-Jahr: | 2021 |
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| Jahr: | 16 February 2021 |
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| Umfang: | 20 S. |
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| Fussnoten: | Gesehen am 27.04.2021 |
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| Titel Quelle: | Enthalten in: Nature Communications |
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| Ort Quelle: | [London] : Springer Nature, 2010 |
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| Jahr Quelle: | 2021 |
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| Band/Heft Quelle: | 12(2021), Artikel-ID 1072, Seite 1-20 |
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| ISSN Quelle: | 2041-1723 |
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| Abstract: | In addition to nucleosomes, chromatin contains non-histone chromatin-associated proteins, of which the high-mobility group proteins are the most abundant. Chromatin-mediated regulation of transcription involves DNA methylation and histone modifications. However, the order of events and the precise function of high-mobility group proteins during transcription initiation remain unclear. Here we show that high-mobility group AT-hook 2 protein (HMGA2) induces DNA nicks at the transcription start site, which are required by the histone chaperone FACT complex to incorporate nucleosomes containing the histone variant H2A.X. Further, phosphorylation of H2A.X at S139 (γ-H2AX) is required for repair-mediated DNA demethylation and transcription activation. The relevance of these findings is demonstrated within the context of TGFB1 signaling and idiopathic pulmonary fibrosis, suggesting therapies against this lethal disease. Our data support the concept that chromatin opening during transcriptional initiation involves intermediates with DNA breaks that subsequently require DNA repair mechanisms to ensure genome integrity. |
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| DOI: | doi:10.1038/s41467-021-21227-y |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1038/s41467-021-21227-y |
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| | Volltext: https://www.nature.com/articles/s41467-021-21227-y |
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| | DOI: https://doi.org/10.1038/s41467-021-21227-y |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| K10plus-PPN: | 1756011419 |
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| Verknüpfungen: | → Zeitschrift |
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Positioning of nucleosomes containing γ-H2AX precedes active DNA demethylation and transcription initiation / Dobersch, Stephanie [VerfasserIn]; 16 February 2021 (Online-Ressource)
