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Status: Bibliographieeintrag

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Verfasst von:Peixoto, Joana [VerfasserIn]   i
 Janaki-Raman, Sudha [VerfasserIn]   i
 Schlicker, Lisa [VerfasserIn]   i
 Schmitz, Werner [VerfasserIn]   i
 Walz, Susanne [VerfasserIn]   i
 Winkelkotte, Alina M. [VerfasserIn]   i
 Herold-Mende, Christel [VerfasserIn]   i
 Soares, Paula [VerfasserIn]   i
 Schulze, Almut [VerfasserIn]   i
 Lima, Jorge [VerfasserIn]   i
Titel:Integrated metabolomics and transcriptomics analysis of monolayer and neurospheres from established glioblastoma cell lines
Verf.angabe:Joana Peixoto, Sudha Janaki-Raman, Lisa Schlicker, Werner Schmitz, Susanne Walz, Alina M. Winkelkotte, Christel Herold-Mende, Paula Soares, Almut Schulze and Jorge Lima
E-Jahr:2021
Jahr:16 March 2021
Umfang:23 S.
Fussnoten:Gesehen am 28.04.2021
Titel Quelle:Enthalten in: Cancers
Ort Quelle:Basel : MDPI, 2009
Jahr Quelle:2021
Band/Heft Quelle:13(2021), 6, Artikel-ID 1327, Seite 1-23
ISSN Quelle:2072-6694
Abstract:Altered metabolic processes contribute to carcinogenesis by modulating proliferation, survival and differentiation. Tumours are composed of different cell populations, with cancer stem-like cells being one of the most prominent examples. This specific pool of cells is thought to be responsible for cancer growth and recurrence and plays a particularly relevant role in glioblastoma (GBM), the most lethal form of primary brain tumours. Here, we have analysed the transcriptome and metabolome of an established GBM cell line (U87) and a patient-derived GBM stem-like cell line (NCH644) exposed to neurosphere or monolayer culture conditions. By integrating transcriptome and metabolome data, we identified key metabolic pathways and gene signatures that are associated with stem-like and differentiated states in GBM cells, and demonstrated that neurospheres and monolayer cells differ substantially in their metabolism and gene regulation. Furthermore, arginine biosynthesis was identified as the most significantly regulated pathway in neurospheres, although individual nodes of this pathway were distinctly regulated in the two cellular systems. Neurosphere conditions, as opposed to monolayer conditions, cause a transcriptomic and metabolic rewiring that may be crucial for the regulation of stem-like features, where arginine biosynthesis may be a key metabolic pathway. Additionally, TCGA data from GBM patients showed significant regulation of specific components of the arginine biosynthesis pathway, providing further evidence for the importance of this metabolic pathway in GBM.
DOI:doi:10.3390/cancers13061327
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext ; Verlag: https://doi.org/10.3390/cancers13061327
 Volltext: https://www.mdpi.com/2072-6694/13/6/1327
 DOI: https://doi.org/10.3390/cancers13061327
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:arginine metabolism
 glioblastoma
 metabolome
 monolayer
 neurospheres
 transcriptome
K10plus-PPN:1756033323
Verknüpfungen:→ Zeitschrift

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