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| Verfasst von: | Schmidt, Stefan [VerfasserIn]  |
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| | Linge, Annett [VerfasserIn] |
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| | Grosser, Marianne [VerfasserIn] |
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| | Lohaus, Fabian [VerfasserIn] |
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| | Gudziol, Volker [VerfasserIn] |
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| | Nowak, Alexander [VerfasserIn] |
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| | Tinhofer, Ingeborg [VerfasserIn] |
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| | Budach, Volker [VerfasserIn] |
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| | Sak, Ali [VerfasserIn] |
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| | Stuschke, Martin [VerfasserIn] |
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| | Balermpas, Panagiotis [VerfasserIn] |
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| | Rödel, Claus [VerfasserIn] |
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| | Schäfer, Henning [VerfasserIn] |
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| | Grosu, Anca-Ligia [VerfasserIn] |
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| | Abdollahi, Amir [VerfasserIn] |
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| | Debus, Jürgen [VerfasserIn] |
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| | Ganswindt, Ute [VerfasserIn] |
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| | Belka, Claus [VerfasserIn] |
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| | Pigorsch, Steffi [VerfasserIn] |
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| | Combs, Stephanie E. [VerfasserIn] |
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| | Mönnich, David [VerfasserIn] |
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| | Zips, Daniel [VerfasserIn] |
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| | Baretton, Gustavo B. [VerfasserIn] |
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| | Buchholz, Frank [VerfasserIn] |
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| | Baumann, Michael [VerfasserIn] |
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| | Krause, Mechthild [VerfasserIn] |
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| | Löck, Steffen [VerfasserIn] |
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| Titel: | Comparison of GeneChip, nCounter, and real-time PCR-based gene expressions predicting locoregional tumor control after primary and postoperative radiochemotherapy in head and neck squamous cell carcinoma |
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| Verf.angabe: | Stefan Schmidt, Annett Linge, Marianne Grosser, Fabian Lohaus, Volker Gudziol, Alexander Nowak, Ingeborg Tinhofer, Volker Budach, Ali Sak, Martin Stuschke, Panagiotis Balermpas, Claus Rödel, Henning Schäfer, Anca-Ligia Grosu, Amir Abdollahi, Jürgen Debus, Ute Ganswindt, Claus Belka, Steffi Pigorsch, Stephanie E. Combs, David Mönnich, Daniel Zips, Gustavo B. Baretton, Frank Buchholz, Michael Baumann, Mechthild Krause, and Steffen Löck for the German Cancer Consortium Radiation Oncology Group |
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| E-Jahr: | 2020 |
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| Jahr: | 2 April 2020 |
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| Umfang: | 10 S. |
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| Fussnoten: | Gesehen am 29.04.2021 |
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| Titel Quelle: | Enthalten in: The journal of molecular diagnostics |
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| Ort Quelle: | Amsterdam [u.a.] : Elsevier, 1999 |
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| Jahr Quelle: | 2020 |
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| Band/Heft Quelle: | 22(2020), 6 vom: Juni, Seite 801-810 |
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| ISSN Quelle: | 1943-7811 |
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| Abstract: | This article compares the expression and applicability of biomarkers, from single genes and gene signatures, identified in patients with locally advanced head and neck squamous cell carcinoma using the GeneChip Human Transcriptome Array 2.0, nCounter, and real-time PCR analyses. Two multicenter, retrospective cohorts of patients with head and neck squamous cell carcinoma from the German Cancer Consortium Radiation Oncology Group who received postoperative radiochemotherapy or primary radiochemotherapy were considered. Real-time PCR was performed for a limited number of 38 genes of the cohort who received postoperative radiochemotherapy only. Correlations between the methods were evaluated by the Spearman rank correlation coefficient. Patients were stratified based on the expression of putative cancer stem cell markers, hypoxia-associated gene signatures, and a previously developed seven-gene signature. Locoregional tumor control was compared between these patient subgroups using log-rank tests. Gene expressions obtained from nCounter analyses were moderately correlated to GeneChip analyses (median ρ = approximately 0.68). A higher correlation was obtained between nCounter analyses and real-time PCR (median ρ = 0.84). Significant associations with locoregional tumor control were observed for most of the considered biomarkers evaluated by GeneChip and nCounter analyses. In general, all applied biomarkers (single genes and gene signatures) classified approximately 70% to 85% of the patients similarly. Overall, gene signatures seem to be more robust and had a better transferability among different measurement methods. |
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| DOI: | doi:10.1016/j.jmoldx.2020.03.005 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1016/j.jmoldx.2020.03.005 |
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| | Volltext: https://www.sciencedirect.com/science/article/pii/S1525157820300763 |
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| | DOI: https://doi.org/10.1016/j.jmoldx.2020.03.005 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| K10plus-PPN: | 1756557020 |
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| Verknüpfungen: | → Zeitschrift |
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Comparison of GeneChip, nCounter, and real-time PCR-based gene expressions predicting locoregional tumor control after primary and postoperative radiochemotherapy in head and neck squamous cell carcinoma / Schmidt, Stefan [VerfasserIn]; 2 April 2020 (Online-Ressource)
