Alterations in rat brain proteins after desflurane anesthesia

• Verfasst von: Fütterer, Carsten Dietmar [VerfasserIn]
• Verfasst von: Maurer, Martin H. [VerfasserIn]
• Verfasst von: Schmitt, Anne [VerfasserIn]
• Verfasst von: Feldmann, Robert Enrico [VerfasserIn]
• Verfasst von: Kuschinsky, Wolfgang [VerfasserIn]
• Verfasst von: Waschke, Klaus F. [VerfasserIn]
• Titel: Alterations in rat brain proteins after desflurane anesthesia
• Verf.angabe: Carsten D. Fütterer, Martin H. Maurer, Anne Schmitt, Robert E. Feldmann, Wolfgang Kuschinsky, Klaus F. Waschke
• E-Jahr: 2004
• Jahr: [Feb 2004]
• Umfang: 7 S.
• Fussnoten: Gesehen am 05.05.2021
• Titel Quelle: Enthalten in: Anesthesiology
• Ort Quelle: Hagerstown, Md. : Lippincott Williams & Wilkins, 1940
• Jahr Quelle: 2004
• Band/Heft Quelle: 100(2004), 2 vom: Feb., Seite 302-308
• ISSN Quelle: 1528-1175
• DOI: doi:10.1097/00000542-200402000-00019
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 175712621X

Abstract

Volatile anesthetics disappear from an organism after the end of anesthesia. Whether changes of protein expression persist in the brain for a longer period is not known. This study investigates the question of whether the expression of proteins is altered in the rat brain after the end of desflurane anesthesia.Three groups (n = 12 each) of rats were anesthetized with 5.7% desflurane in air for 3 h. Brains were removed directly after anesthesia, 24 h after anesthesia, or 72 h after anesthesia. Two additional groups (n = 12 each) served as naive conscious controls, in which the brains were removed without previous anesthesia 3 or 72 h after the start of the experiment. Cytosolic proteins were isolated. A proteome-wide study was performed, based on two-dimensional gel electrophoresis and mass spectrometry.Compared with conscious controls, significant (P < 0.05) increase/decrease was found: 3 h of anesthesia, 5/2 proteins; 24 h after anesthesia, 13/1 proteins; 72 h after anesthesia, 6/4 proteins. The overall changes in protein expression as quantified by the induction factor ranged from -1.67 (decrease to 60%) to 1.79 (increase by 79%) compared with the controls (100%). Some of these regulated proteins play a role in vesicle transport and metabolism.Desflurane anesthesia produces changes in cytosolic protein expression up to 72 h after anesthesia in the rat brain, indicating yet unknown persisting effects.