Status: Bibliographieeintrag
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| Verfasst von: | Kabsch, Kirsten [VerfasserIn]  |
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| | Mossadegh, Nina [VerfasserIn] |
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| | Kohl, Annette [VerfasserIn] |
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| | Komposch, Gerda [VerfasserIn] |
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| | Schenkel, Johannes [VerfasserIn] |
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| | Alonso, Angel [VerfasserIn] |
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| | Tomakidi, Pascal [VerfasserIn] |
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| Titel: | The HPV-16 E5 protein Inhibits TRAIL- and FasL-Mediated apoptosis in human keratinocyte raft cultures |
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| Verf.angabe: | Kirsten Kabsch, Nina Mossadegh, Annette Kohl, Gerda Komposch, Johannes Schenkel, Angel Alonso, Pascal Tomakidi |
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| E-Jahr: | 2004 |
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| Jahr: | March 26, 2004 |
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| Umfang: | 9 S. |
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| Teil: | volume:47 |
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| | year:2004 |
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| | number:1 |
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| | pages:48-56 |
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| | extent:9 |
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| Fussnoten: | Gesehen am 05.05.2021 |
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| Titel Quelle: | Enthalten in: Intervirology |
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| Ort Quelle: | Basel : Karger, 1973 |
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| Jahr Quelle: | 2004 |
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| Band/Heft Quelle: | 47(2004), 1, Seite 48-56 |
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| ISSN Quelle: | 1423-0100 |
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| Abstract: | By using raft cultures of the polyclonal HaCaT cell lines stably transfected either with E5 (HaCaT/E5) or the empty vector (HaCaT/pMSG) as reference, we investigated the effect of the human papillomavirus type 16 (HPV-16) E5 protein on apoptosis. In comparison to conventional monolayer cultures this model system allows analysis of apoptosis under more tissue-like conditions by mimicking the stratified organization of a normal surface epithelium. Apoptosis was triggered either by FasL or TRAIL. Execution of the death program was checked at early and late stages by monitoring procaspase-3 cleavage and DNA fragmentation, respectively. Rafts of E5-expressing keratinocytes were completely protected from apoptosis and showed a background of apoptotic cells as low as the untreated cultures. In contrast, the HaCaT/pMSG cultures revealed a dramatic increase in apoptotic cells upon ligand treatment throughout the epithelial compartment. We conclude that the presence of the HPV-16 E5 protein in our tissue-like model prevents FasL- or TRAIL-mediated apoptosis. |
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| DOI: | doi:10.1159/000076642 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1159/000076642 |
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| | Volltext: https://www.karger.com/Article/FullText/76642 |
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| | DOI: https://doi.org/10.1159/000076642 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| K10plus-PPN: | 1757128719 |
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| Verknüpfungen: | → Zeitschrift |
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¬The¬ HPV-16 E5 protein Inhibits TRAIL- and FasL-Mediated apoptosis in human keratinocyte raft cultures / Kabsch, Kirsten [VerfasserIn]; March 26, 2004 (Online-Ressource)
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