The proteome of human brain microdialysate

• Verfasst von: Maurer, Martin H. [VerfasserIn]
• Verfasst von: Berger, Christian [VerfasserIn]
• Verfasst von: Wolf, Margit [VerfasserIn]
• Verfasst von: Fütterer, Carsten Dietmar [VerfasserIn]
• Verfasst von: Feldmann, Robert Enrico [VerfasserIn]
• Verfasst von: Schwab, Stefan [VerfasserIn]
• Verfasst von: Kuschinsky, Wolfgang [VerfasserIn]
• Titel: The proteome of human brain microdialysate
• Verf.angabe: Martin H. Maurer, Christian Berger, Margit Wolf, Carsten D. Fütterer, Robert E. Feldmann, Stefan Schwab and Wolfgang Kuschinsky
• E-Jahr: 2003
• Jahr: 14 December 2003
• Umfang: 9 S.
• Fussnoten: Gesehen am 05.05.2021
• Titel Quelle: Enthalten in: Proteome science
• Ort Quelle: London : BioMed Central, 2003
• Jahr Quelle: 2003
• Band/Heft Quelle: 1(2003), Artikel-ID 7, Seite 1-9
• ISSN Quelle: 1477-5956
• DOI: doi:10.1186/1477-5956-1-7
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Brain Edema
• Sach-SW: Contralateral Hemisphere
• Sach-SW: Microdialysis Probe
• Sach-SW: Protein Spot
• Sach-SW: Stroke Patient
• K10plus-PPN: 175713011X

Abstract

Cerebral microdialysis has been established as a monitoring tool in neurocritically ill patients suffering from severe stroke. The technique allows to sample small molecules in the brain tissue for subsequent biochemical analysis. In this study, we investigated the proteomic profile of human cerebral microdialysate and if the identified proteins might be useful predictors for disease characteristics in stroke for tissue at risk in the contralateral hemisphere. We analysed cerebral protein expression in microdialysate from three stroke patients sampled from the hemisphere contralateral to the lesion. Using a proteomic approach based on two-dimensional gel electrophoresis and subsequent mass spectrometry, we created a protein map for the global protein expression pattern of human microdialyste.