| |  Online-Ressource |
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| Verfasst von: | Tolkach, Iurii [VerfasserIn]  |
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| | Zarbl, Romina [VerfasserIn] |
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| | Bauer, Simone [VerfasserIn] |
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| | Ritter, Manuel [VerfasserIn] |
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| | Ellinger, Jörg [VerfasserIn] |
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| | Hauser, Stephan [VerfasserIn] |
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| | Hüser, Laura [VerfasserIn] |
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| | Klauck, Sabine [VerfasserIn] |
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| | Altevogt, Peter [VerfasserIn] |
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| | Sültmann, Holger [VerfasserIn] |
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| | Dietrich, Dimo [VerfasserIn] |
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| | Kristiansen, Glen [VerfasserIn] |
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| Titel: | DNA promoter methylation and ERG regulate the expression of CD24 in prostate cancer |
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| Verf.angabe: | Yuri Tolkach, Romina Zarbl, Simone Bauer, Manuel Ritter, Jörg Ellinger, Stephan Hauser, Laura Hüser, Sabine M. Klauck, Peter Altevogt, Holger Sültmann, Dimo Dietrich, and Glen Kristiansen |
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| E-Jahr: | 2021 |
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| Jahr: | 22 January 2021 |
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| Umfang: | 13 S. |
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| Fussnoten: | Gesehen am 07.05.2021 |
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| Titel Quelle: | Enthalten in: The American journal of pathology |
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| Ort Quelle: | New York [u.a.] : Elsevier, 1925 |
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| Jahr Quelle: | 2021 |
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| Band/Heft Quelle: | 191(2021), 4, Seite 618-630 |
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| ISSN Quelle: | 1525-2191 |
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| Abstract: | CD24 is overexpressed in many human cancers and is a driver of tumor progression. Herein, molecular mechanisms leading to up-regulation of CD24 in prostate cancer were studied. DNA methylation of the CD24 gene promoter at four loci using quantitative methylation-specific PCR was evaluated. Expression of CD24 in tumor tissues was studied by immunohistochemistry. To corroborate the results in vitro, ERG-inducible LNCaP TMPRSS2:ERG (T2E) cells and luciferase promoter assays were used. DNA methylation of the CD24 promoter was significantly higher in tumors than in benign tissue and was associated with biochemical recurrence-free survival, tumor grade, and stage. CD24 mRNA and protein expression were significantly higher in T2E-positive, ERG-overexpressing, and/or PTEN-deficient cases. Higher levels of CD24 protein expression conferred shorter biochemical recurrence-free survival, and these observations were confirmed using The Cancer Genome Atlas prostate adenocarcinoma data. In silico analysis of the CD24 promoter revealed an ERG binding site in between the DNA methylation sites. ERG overexpression led to a strong induction of CD24 mRNA and protein expression. Luciferase promoter assays using the wild-type and mutated ERG binding site within the CD24 promoter showed ERG-dependent activation. Collectively, our results suggest that promoter DNA methylation of the CD24 gene and T2E fusion status are factors involved in the up-regulation of CD24 in patients with prostate cancer. |
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| DOI: | doi:10.1016/j.ajpath.2020.12.014 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1016/j.ajpath.2020.12.014 |
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| | Volltext: https://www.sciencedirect.com/science/article/pii/S0002944021000092 |
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| | DOI: https://doi.org/10.1016/j.ajpath.2020.12.014 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| K10plus-PPN: | 1757489584 |
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| Verknüpfungen: | → Zeitschrift |
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DNA promoter methylation and ERG regulate the expression of CD24 in prostate cancer / Tolkach, Iurii [VerfasserIn]; 22 January 2021 (Online-Ressource)
