| |  Online-Ressource |
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| Verfasst von: | Hierlmeier, Thomas [VerfasserIn]  |
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| | Merl, Juliane [VerfasserIn] |
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| | Sauert, Martina [VerfasserIn] |
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| | Perez-Fernandez, Jorge [VerfasserIn] |
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| | Schultz, Patrick [VerfasserIn] |
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| | Bruckmann, Astrid [VerfasserIn] |
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| | Hamperl, Stephan [VerfasserIn] |
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| | Ohmayer, Uli [VerfasserIn] |
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| | Rachel, Reinhard [VerfasserIn] |
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| | Jacob, Anja [VerfasserIn] |
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| | Hergert, Kristin [VerfasserIn] |
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| | Deutzmann, Rainer [VerfasserIn] |
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| | Griesenbeck, Joachim [VerfasserIn] |
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| | Hurt, Ed [VerfasserIn] |
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| | Milkereit, Philipp [VerfasserIn] |
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| | Baßler, Jochen [VerfasserIn] |
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| | Tschochner, Herbert [VerfasserIn] |
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| Titel: | Rrp5p, Noc1p and Noc2p form a protein module which is part of early large ribosomal subunit precursors in S. cerevisiae |
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| Verf.angabe: | Thomas Hierlmeier, Juliane Merl, Martina Sauert, Jorge Perez-Fernandez, Patrick Schultz, Astrid Bruckmann, Stephan Hamperl, Uli Ohmayer, Reinhard Rachel, Anja Jacob, Kristin Hergert, Rainer Deutzmann, Joachim Griesenbeck, Ed Hurt, Philipp Milkereit, Jochen Baßler and Herbert Tschochner |
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| Jahr: | 2013 |
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| Jahr des Originals: | 2012 |
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| Umfang: | 20 S. |
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| Teil: | volume:41 |
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| | year:2013 |
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| | number:2 |
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| | pages:1191-1210 |
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| | extent:20 |
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| Fussnoten: | Gesehen am 10.05.2021 ; Published: 02 December 2012 |
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| Titel Quelle: | Enthalten in: Nucleic acids research |
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| Ort Quelle: | Oxford : Oxford Univ. Press, 1974 |
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| Jahr Quelle: | 2013 |
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| Band/Heft Quelle: | 41(2013), 2, Seite 1191-1210 |
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| ISSN Quelle: | 1362-4962 |
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| Abstract: | Eukaryotic ribosome biogenesis requires more than 150 auxiliary proteins, which transiently interact with pre-ribosomal particles. Previous studies suggest that several of these biogenesis factors function together as modules. Using a heterologous expression system, we show that the large ribosomal subunit (LSU) biogenesis factor Noc1p of Saccharomyces cerevisiae can simultaneously interact with the LSU biogenesis factor Noc2p and Rrp5p, a factor required for biogenesis of the large and the small ribosomal subunit. Proteome analysis of RNA polymerase-I-associated chromatin and chromatin immunopurification experiments indicated that all members of this protein module and a specific set of LSU biogenesis factors are co-transcriptionally recruited to nascent ribosomal RNA (rRNA) precursors in yeast cells. Further ex vivo analyses showed that all module members predominantly interact with early pre-LSU particles after the initial pre-rRNA processing events have occurred. In yeast strains depleted of Noc1p, Noc2p or Rrp5p, levels of the major LSU pre-rRNAs decreased and the respective other module members were associated with accumulating aberrant rRNA fragments. Therefore, we conclude that the module exhibits several binding interfaces with pre-ribosomes. Taken together, our results suggest a co- and post-transcriptional role of the yeast Rrp5p-Noc1p-Noc2p module in the structural organization of early LSU precursors protecting them from non-productive RNase activity. |
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| DOI: | doi:10.1093/nar/gks1056 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1093/nar/gks1056 |
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| | DOI: https://doi.org/10.1093/nar/gks1056 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| K10plus-PPN: | 1757579729 |
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| Verknüpfungen: | → Zeitschrift |
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Rrp5p, Noc1p and Noc2p form a protein module which is part of early large ribosomal subunit precursors in S. cerevisiae / Hierlmeier, Thomas [VerfasserIn]; 2013 (Online-Ressource)
