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Verfasst von:Hierlmeier, Thomas [VerfasserIn]   i
 Merl, Juliane [VerfasserIn]   i
 Sauert, Martina [VerfasserIn]   i
 Perez-Fernandez, Jorge [VerfasserIn]   i
 Schultz, Patrick [VerfasserIn]   i
 Bruckmann, Astrid [VerfasserIn]   i
 Hamperl, Stephan [VerfasserIn]   i
 Ohmayer, Uli [VerfasserIn]   i
 Rachel, Reinhard [VerfasserIn]   i
 Jacob, Anja [VerfasserIn]   i
 Hergert, Kristin [VerfasserIn]   i
 Deutzmann, Rainer [VerfasserIn]   i
 Griesenbeck, Joachim [VerfasserIn]   i
 Hurt, Ed [VerfasserIn]   i
 Milkereit, Philipp [VerfasserIn]   i
 Baßler, Jochen [VerfasserIn]   i
 Tschochner, Herbert [VerfasserIn]   i
Titel:Rrp5p, Noc1p and Noc2p form a protein module which is part of early large ribosomal subunit precursors in S. cerevisiae
Verf.angabe:Thomas Hierlmeier, Juliane Merl, Martina Sauert, Jorge Perez-Fernandez, Patrick Schultz, Astrid Bruckmann, Stephan Hamperl, Uli Ohmayer, Reinhard Rachel, Anja Jacob, Kristin Hergert, Rainer Deutzmann, Joachim Griesenbeck, Ed Hurt, Philipp Milkereit, Jochen Baßler and Herbert Tschochner
Jahr:2013
Jahr des Originals:2012
Umfang:20 S.
Teil:volume:41
 year:2013
 number:2
 pages:1191-1210
 extent:20
Fussnoten:Gesehen am 10.05.2021 ; Published: 02 December 2012
Titel Quelle:Enthalten in: Nucleic acids research
Ort Quelle:Oxford : Oxford Univ. Press, 1974
Jahr Quelle:2013
Band/Heft Quelle:41(2013), 2, Seite 1191-1210
ISSN Quelle:1362-4962
Abstract:Eukaryotic ribosome biogenesis requires more than 150 auxiliary proteins, which transiently interact with pre-ribosomal particles. Previous studies suggest that several of these biogenesis factors function together as modules. Using a heterologous expression system, we show that the large ribosomal subunit (LSU) biogenesis factor Noc1p of Saccharomyces cerevisiae can simultaneously interact with the LSU biogenesis factor Noc2p and Rrp5p, a factor required for biogenesis of the large and the small ribosomal subunit. Proteome analysis of RNA polymerase-I-associated chromatin and chromatin immunopurification experiments indicated that all members of this protein module and a specific set of LSU biogenesis factors are co-transcriptionally recruited to nascent ribosomal RNA (rRNA) precursors in yeast cells. Further ex vivo analyses showed that all module members predominantly interact with early pre-LSU particles after the initial pre-rRNA processing events have occurred. In yeast strains depleted of Noc1p, Noc2p or Rrp5p, levels of the major LSU pre-rRNAs decreased and the respective other module members were associated with accumulating aberrant rRNA fragments. Therefore, we conclude that the module exhibits several binding interfaces with pre-ribosomes. Taken together, our results suggest a co- and post-transcriptional role of the yeast Rrp5p-Noc1p-Noc2p module in the structural organization of early LSU precursors protecting them from non-productive RNase activity.
DOI:doi:10.1093/nar/gks1056
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext: https://doi.org/10.1093/nar/gks1056
 DOI: https://doi.org/10.1093/nar/gks1056
Datenträger:Online-Ressource
Sprache:eng
K10plus-PPN:1757579729
Verknüpfungen:→ Zeitschrift

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