| |  Online-Ressource |
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| Verfasst von: | Ehrhardt, Manuela [VerfasserIn]  |
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| | Möck, Marion [VerfasserIn] |
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| | Haefeli, Walter E. [VerfasserIn] |
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| | Mikus, Gerd [VerfasserIn] |
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| | Burhenne, Jürgen [VerfasserIn] |
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| Titel: | Monitoring of lopinavir and ritonavir in peripheral blood mononuclear cells, plasma, and ultrafiltrate using a selective and highly sensitive LC/MS/MS assay |
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| Verf.angabe: | Manuela Ehrhardt, Marion Möck, Walter E. Haefeli, Gerd Mikus, Jürgen Burhenne |
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| Jahr: | 2007 |
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| Jahr des Originals: | 2006 |
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| Umfang: | 10 S. |
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| Teil: | volume:850 |
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| | year:2007 |
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| | number:1 |
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| | pages:249-258 |
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| | extent:10 |
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| Fussnoten: | Online 11 December 2006 ; Gesehen am 12.05.2021 |
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| Titel Quelle: | Enthalten in: Journal of chromatography / B |
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| Ort Quelle: | New York, NY [u.a.] : Science Direct, 1977 |
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| Jahr Quelle: | 2007 |
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| Band/Heft Quelle: | 850(2007), 1, Seite 249-258 |
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| ISSN Quelle: | 1873-376X |
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| Abstract: | For the determination of the HIV protease inhibitors lopinavir and ritonavir in human plasma, plasma ultrafiltrate, and peripheral blood mononuclear cells (PBMCs) a highly sensitive and selective method has been developed, validated, and applied to samples of a healthy volunteer. BD Vacutainer® CPT™ and Amicon Centriplus® centrifugal filter devices were used for separation of PBMCs and for ultrafiltrate generation, respectively. After liquid/liquid-extraction extracts were chromatographed isocratically within 6min on a Jupiter Proteo column. The drugs were quantified using 2H5-saquinavir as internal standard and electrospray tandem mass spectrometry in the selected reaction monitoring mode. Limits of quantification for both analytes were 4.0ng/mL in plasma, 0.2ng/mL in ultrafiltrate, and 0.1ng/cell pellet (∼3×106 cells) in PBMCs. The calibration ranges were linear over more than three logs with an over-all accuracy varying between 98.7% and 111.5% and an over-all precision ranging from 6.2% to 14.0% (SD batch-to-batch). After a regular oral dose of Kaletra® (400mg lopinavir, 100mg ritonavir) analyte concentrations were detectable over a full dosing interval in plasma, ultrafiltrate, and PBMCs. The method is well suited for monitoring of free and total plasma, and intracellular lopinavir/ritonavir concentrations in samples from clinical trials. |
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| DOI: | doi:10.1016/j.jchromb.2006.11.037 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1016/j.jchromb.2006.11.037 |
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| | Volltext: https://www.sciencedirect.com/science/article/pii/S1570023206009925 |
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| | DOI: https://doi.org/10.1016/j.jchromb.2006.11.037 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Kaletra |
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| | LC/MS/MS |
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| | Lopinavir |
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| | PBMC |
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| | Protein binding |
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| | Ritonavir |
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| K10plus-PPN: | 1757720391 |
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| Verknüpfungen: | → Zeitschrift |
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Monitoring of lopinavir and ritonavir in peripheral blood mononuclear cells, plasma, and ultrafiltrate using a selective and highly sensitive LC/MS/MS assay / Ehrhardt, Manuela [VerfasserIn]; 2007 (Online-Ressource)
