| |  Online-Ressource |
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| Verfasst von: | Bismar, Hanadi [VerfasserIn]  |
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| | Klöppinger, T [VerfasserIn] |
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| | Schuster, Elke Maria [VerfasserIn] |
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| | Balbach, S [VerfasserIn] |
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| | Diel, Ingo J. [VerfasserIn] |
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| | Ziegler, Reinhard [VerfasserIn] |
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| | Pfeilschifter, Johannes [VerfasserIn] |
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| Titel: | Transforming growth factor β (TGF-β) levels in the conditioned media of human bone cells |
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| Titelzusatz: | relationship to donor age, bone volume, and concentration of TGF-β in human bone matrix in vivo |
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| Verf.angabe: | H. Bismar, T. Klöppinger, E.M. Schuster, S. Balbach, I. Diel, R. Ziegler, and J. Pfeilschifter |
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| E-Jahr: | 1999 |
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| Jahr: | 24 May 1999 |
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| Umfang: | 5 S. |
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| Teil: | volume:24 |
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| | year:1999 |
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| | number:6 |
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| | pages:565-569 |
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| | extent:5 |
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| Fussnoten: | Gesehen am 14.05.2021 |
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| Titel Quelle: | Enthalten in: Bone |
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| Ort Quelle: | Amsterdam [u.a.] : Elsevier Science, 1985 |
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| Jahr Quelle: | 1999 |
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| Band/Heft Quelle: | 24(1999), 6, Seite 565-569 |
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| ISSN Quelle: | 1873-2763 |
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| Abstract: | Transforming growth factor-β (TGF-β) is thought to play an important role in human bone remodeling. In the present study, we examined constitutive differences in TGF-β levels in primary bone cell cultures from the iliac crest of 112 women, aged 28-79 years. TGF-β1 was the major TGF-β isoform in the conditioned media, as determined by neutralizing TGF-β activity with specific antibodies against TGF-β1-3 in the mink lung cell bioassay, and by enzyme-linked immunoassay (ELISA). TGF-β1 levels in the conditioned media did not change with donor age. There was a lack of association between TGF-β levels in vitro and the concentration of matrix-associated TGF-β in vivo. TGF-β1 levels failed to be associated with the local trabecular bone volume in the complete study population (r = +0.15, p = 0.16, n = 89). A significant association between TGF-β1 levels and bone volume was present in premenopausal women (r = +0.39, p = 0.02, n = 33), but was largely accounted for by the two samples with the highest TGF-β concentrations. In conclusion, our data suggest that TGF-β1 is the major TGF-β isoform produced by human bone cells in vitro, and that the constitutive secretion of TGF-β by bone cells does not change with age. Whether constitutive differences in TGF-β secretion may be a determinant of human bone mass remains to be clarified in further studies. |
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| DOI: | doi:10.1016/S8756-3282(99)00082-4 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://doi.org/10.1016/S8756-3282(99)00082-4 |
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| | Volltext: https://www.sciencedirect.com/science/article/pii/S8756328299000824 |
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| | DOI: https://doi.org/10.1016/S8756-3282(99)00082-4 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Aging |
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| | Bone matrix |
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| | Bone volume |
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| | Conditioned medium |
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| | Human bone cells |
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| | Transforming growth factor-β |
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| K10plus-PPN: | 1757780963 |
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| Verknüpfungen: | → Zeitschrift |
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Transforming growth factor β (TGF-β) levels in the conditioned media of human bone cells / Bismar, Hanadi [VerfasserIn]; 24 May 1999 (Online-Ressource)
