| |  Online-Ressource |
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| Verfasst von: | Martin, Praxedis Sina [VerfasserIn]  |
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| | Pardo, Julián [VerfasserIn] |
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| | Schill, Natalie [VerfasserIn] |
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| | Jöckel, Lars [VerfasserIn] |
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| | Berg, Matthias [VerfasserIn] |
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| | Froelich, Christopher J. [VerfasserIn] |
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| | Wallich, Reinhard [VerfasserIn] |
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| | Simon, Markus M. [VerfasserIn] |
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| Titel: | Granzyme B-induced and caspase 3-dependent cleavage of gelsolin by mouse cytotoxic T cells modifies cytoskeleton dynamics |
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| Verf.angabe: | Praxedis Martin, Julián Pardo, Natalie Schill, Lars Jöckel, Matthias Berg, Christopher J. Froelich, Reinhard Wallich, and Markus M. Simon |
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| Jahr: | 2010 |
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| Umfang: | 10 S. |
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| Teil: | volume:285 |
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| | year:2010 |
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| | number:24 |
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| | pages:18918-18927 |
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| | extent:10 |
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| Fussnoten: | Gesehen am 17.05.2021 |
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| Titel Quelle: | Enthalten in: The journal of biological chemistry |
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| Ort Quelle: | Bethesda, Md. : Soc., 1905 |
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| Jahr Quelle: | 2010 |
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| Band/Heft Quelle: | 285(2010), 24, Seite 18918-18927 |
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| ISSN Quelle: | 1083-351X |
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| Abstract: | Granule-associated perforin and granzymes (gzms) are key effector molecules of cytotoxic T lymphocytes (Tc cells) and natural killer cells and play a critical role in the control of intracellular pathogens and cancer. Based on the notion that many gzms, including A, B, C, K, H, and M exhibit cytotoxic activity in vitro, all gzms are believed to serve a similar function in vivo. However, more recent evidence supports the concept that gzms are not unidimensional but, rather, possess non-cytotoxic potential, including stimulation of pro-inflammatory cytokines and anti-viral activities. The present study shows that isolated mouse gzmB cleaves the actin-severing mouse protein, cytoplasmic gelsolin (c-gelsolin) in vitro. However, when delivered to intact target cells by ex vivo immune Tc cells, gzmB mediates c-gelsolin proteolysis via activation of caspases 3/7. The NH(2)-terminal c-gelsolin fragment generated by either gzmB or caspase 3 in vitro constitutively severs actin filaments without destroying the target cells. The observation that gzmB secreted by Tc cells initiates a caspase cascade that disintegrates the actin cytoskeleton in target cells suggests that this intracellular process may contribute to anti-viral host defense. |
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| DOI: | doi:10.1074/jbc.M109.056028 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://doi.org/10.1074/jbc.M109.056028 |
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| | DOI: https://doi.org/10.1074/jbc.M109.056028 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Animals |
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| | Apoptosis |
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| | Caspase 3 |
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| | CD8-Positive T-Lymphocytes |
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| | Cytoskeleton |
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| | Fibroblasts |
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| | Gelsolin |
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| | Granzymes |
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| | Lymphocytic choriomeningitis virus |
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| | Mice |
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| | Microscopy, Fluorescence |
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| | Models, Biological |
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| | RNA, Messenger |
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| | T-Lymphocytes, Cytotoxic |
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| | Transcription, Genetic |
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| K10plus-PPN: | 1757904239 |
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| Verknüpfungen: | → Zeitschrift |
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Granzyme B-induced and caspase 3-dependent cleavage of gelsolin by mouse cytotoxic T cells modifies cytoskeleton dynamics / Martin, Praxedis Sina [VerfasserIn]; 2010 (Online-Ressource)
