| |  Online-Ressource |
|---|
| Verfasst von: | Dieterich, Roswitha [VerfasserIn]  |
|---|
| | Hammerschmidt, Claudia [VerfasserIn] |
|---|
| | Richter, Dania [VerfasserIn] |
|---|
| | Skerka, Christine [VerfasserIn] |
|---|
| | Wallich, Reinhard [VerfasserIn] |
|---|
| | Matuschka, Franz-Rainer [VerfasserIn] |
|---|
| | Zipfel, Peter F. [VerfasserIn] |
|---|
| | Kraiczy, Peter [VerfasserIn] |
|---|
| Titel: | Inadequate binding of immune regulator factor H is associated with sensitivity of Borrelia lusitaniae to human complement |
|---|
| Verf.angabe: | Roswitha Dieterich, Claudia Hammerschmidt, Dania Richter, Christine Skerka, Reinhard Wallich, Franz-Rainer Matuschka, Peter F. Zipfel, and Peter Kraiczy |
|---|
| E-Jahr: | 2010 |
|---|
| Jahr: | 7 September 2010 |
|---|
| Umfang: | 10 S. |
|---|
| Fussnoten: | Gesehen am 17.05.2021 |
|---|
| Titel Quelle: | Enthalten in: Infection and immunity |
|---|
| Ort Quelle: | Washington, DC : Soc., 1970 |
|---|
| Jahr Quelle: | 2010 |
|---|
| Band/Heft Quelle: | 78(2010), 11, Seite 4467-4476 |
|---|
| ISSN Quelle: | 1098-5522 |
|---|
| Abstract: | Spirochetes belonging to the Borrelia burgdorferi sensu lato complex differ in resistance to complement-mediated killing by human serum. Here, we characterize complement sensitivity of a panel of B. lusitaniae isolates derived from ticks collected in Germany and Portugal as well as one patient-derived isolate, PoHL. All isolates are highly susceptible to complement-mediated lysis in human serum and activate complement predominantly by the alternative pathway, leading to an increased deposition of complement components C3, C6, and the terminal complement complex. Interestingly, serum-sensitive B. lusitaniae isolates were able to bind immune regulator factor H (CFH), and some strains also bound CFH-related protein 1 (CFHR1) and CFHR2. Moreover, CFH bound to the surface of B. lusitaniae was inefficient in mediating C3b conversion. Furthermore, the identification and characterization of a potential CFH-binding protein, OspE, revealed that this molecule possesses a significantly reduced binding capacity for CFH compared to that of CFH-binding OspE paralogs expressed by various serum-resistant Borrelia species. This finding suggests that a reduced binding capability of CFH is associated with an increased serum sensitivity of B. lusitaniae to human complement. |
|---|
| DOI: | doi:10.1128/IAI.00138-10 |
|---|
| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: https://dx.doi.org/10.1128/IAI.00138-10 |
|---|
| | Volltext: https://iai.asm.org/content/78/11/4467 |
|---|
| | DOI: https://doi.org/10.1128/IAI.00138-10 |
|---|
| Datenträger: | Online-Ressource |
|---|
| Sprache: | eng |
|---|
| Sach-SW: | Animals |
|---|
| | Bacterial Outer Membrane Proteins |
|---|
| | Blood Bactericidal Activity |
|---|
| | Blood Proteins |
|---|
| | Borrelia |
|---|
| | Borrelia Infections |
|---|
| | Complement Activation |
|---|
| | Complement C3b Inactivator Proteins |
|---|
| | Complement Factor H |
|---|
| | Complement Pathway, Alternative |
|---|
| | Germany |
|---|
| | Humans |
|---|
| | Ixodes |
|---|
| | Molecular Sequence Data |
|---|
| | Portugal |
|---|
| | Sequence Analysis, DNA |
|---|
| K10plus-PPN: | 1757915885 |
|---|
| Verknüpfungen: | → Zeitschrift |
|---|
Inadequate binding of immune regulator factor H is associated with sensitivity of Borrelia lusitaniae to human complement / Dieterich, Roswitha [VerfasserIn]; 7 September 2010 (Online-Ressource)
