| Online-Ressource |
Verfasst von: | Aguiló, Juan [VerfasserIn]  |
| Anel, Alberto [VerfasserIn]  |
| Catalán, Elena [VerfasserIn]  |
| Sebastián, Alvaro [VerfasserIn]  |
| Acín-Pérez, Rebeca [VerfasserIn]  |
| Naval, Javier [VerfasserIn]  |
| Wallich, Reinhard [VerfasserIn]  |
| Simon, Markus M. [VerfasserIn]  |
| Pardo, Julián [VerfasserIn]  |
Titel: | Granzyme B of cytotoxic T cells induces extramitochondrial reactive oxygen species production via caspase-dependent NADPH oxidase activation |
Verf.angabe: | Juan I. Aguiló, Alberto Anel, Elena Catalán, Alvaro Sebastián, Rebeca Acín-Pérez, Javier Naval, Reinhard Wallich, Markus M. Simon, Julián Pardo |
E-Jahr: | 2010 |
Jahr: | 02 February 2010 |
Umfang: | 10 S. |
Teil: | volume:88 |
| year:2010 |
| number:5 |
| pages:545-554 |
| extent:10 |
Fussnoten: | Gesehen am 17.05.2021 |
Titel Quelle: | Enthalten in: Immunology & cell biology |
Ort Quelle: | Hoboken, NJ : Wiley, 1987 |
Jahr Quelle: | 2010 |
Band/Heft Quelle: | 88(2010), 5, Seite 545-554 |
ISSN Quelle: | 1440-1711 |
Abstract: | Induction of reactive oxygen species (ROS) is a hallmark of granzyme B (gzmB)-mediated pro-apoptotic processes and target cell death. However, it is unclear to what extent the generated ROS derive from mitochondrial and/or extra-mitochondrial sources. To clarify this point, we have produced a mutant EL4 cell line, termed EL4-rho(0), which lacks mitochondrial DNA, associated with a decreased mitochondrial membrane potential and a defective ROS production through the electron transport chain of oxidative phosphorylation. When incubated with either recombinant gzmB plus streptolysin or ex vivo gzmB(+) cytotoxic T cells, EL4-rho(0) cells showed phosphatydylserine translocation, caspase 3 activation, Bak conformational change, cytochrome c release and apoptotic morphology comparable to EL4 cells. Moreover, EL4-rho(0) cells produced ROS at levels similar to EL4 under these conditions. GzmB-mediated ROS production was almost totally abolished in both cell lines by the pan-caspase inhibitor, Z-VAD-fmk. However, addition of apocynin, a specific inhibitor of nicotinamide adenine dinucleotide phosphate (NADPH) oxidases, led to a significant reduction of ROS production and cell death only in EL4-rho(0) but not EL4 cells. These data suggest that gzmB-induced cell death is accompanied by a caspase-dependent pathway of extra-mitochondrial ROS production, most probably through activation of NADPH oxidase. |
DOI: | doi:10.1038/icb.2010.5 |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://dx.doi.org/10.1038/icb.2010.5 |
| Volltext: https://onlinelibrary.wiley.com/doi/epdf/10.1038/icb.2010.5 |
| DOI: https://doi.org/10.1038/icb.2010.5 |
Datenträger: | Online-Ressource |
Sprache: | eng |
Sach-SW: | Animals |
| Apoptosis |
| Caspases |
| Cell Line |
| Enzyme Activation |
| Granzymes |
| Humans |
| Immunoblotting |
| Mice |
| Mice, Inbred C57BL |
| Mitochondria |
| NADPH Oxidases |
| Reactive Oxygen Species |
| Reverse Transcriptase Polymerase Chain Reaction |
| Signal Transduction |
| T-Lymphocytes, Cytotoxic |
K10plus-PPN: | 1757918639 |
Verknüpfungen: | → Zeitschrift |
Granzyme B of cytotoxic T cells induces extramitochondrial reactive oxygen species production via caspase-dependent NADPH oxidase activation / Aguiló, Juan [VerfasserIn]; 02 February 2010 (Online-Ressource)