| |  Online-Ressource |
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| Verfasst von: | Aguiló, Juan [VerfasserIn]  |
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| | Anel, Alberto [VerfasserIn] |
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| | Catalán, Elena [VerfasserIn] |
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| | Sebastián, Alvaro [VerfasserIn] |
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| | Acín-Pérez, Rebeca [VerfasserIn] |
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| | Naval, Javier [VerfasserIn] |
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| | Wallich, Reinhard [VerfasserIn] |
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| | Simon, Markus M. [VerfasserIn] |
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| | Pardo, Julián [VerfasserIn] |
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| Titel: | Granzyme B of cytotoxic T cells induces extramitochondrial reactive oxygen species production via caspase-dependent NADPH oxidase activation |
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| Verf.angabe: | Juan I. Aguiló, Alberto Anel, Elena Catalán, Alvaro Sebastián, Rebeca Acín-Pérez, Javier Naval, Reinhard Wallich, Markus M. Simon, Julián Pardo |
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| E-Jahr: | 2010 |
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| Jahr: | 02 February 2010 |
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| Umfang: | 10 S. |
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| Teil: | volume:88 |
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| | year:2010 |
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| | number:5 |
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| | pages:545-554 |
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| | extent:10 |
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| Fussnoten: | Gesehen am 17.05.2021 |
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| Titel Quelle: | Enthalten in: Immunology & cell biology |
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| Ort Quelle: | Hoboken, NJ : Wiley, 1987 |
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| Jahr Quelle: | 2010 |
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| Band/Heft Quelle: | 88(2010), 5, Seite 545-554 |
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| ISSN Quelle: | 1440-1711 |
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| Abstract: | Induction of reactive oxygen species (ROS) is a hallmark of granzyme B (gzmB)-mediated pro-apoptotic processes and target cell death. However, it is unclear to what extent the generated ROS derive from mitochondrial and/or extra-mitochondrial sources. To clarify this point, we have produced a mutant EL4 cell line, termed EL4-rho(0), which lacks mitochondrial DNA, associated with a decreased mitochondrial membrane potential and a defective ROS production through the electron transport chain of oxidative phosphorylation. When incubated with either recombinant gzmB plus streptolysin or ex vivo gzmB(+) cytotoxic T cells, EL4-rho(0) cells showed phosphatydylserine translocation, caspase 3 activation, Bak conformational change, cytochrome c release and apoptotic morphology comparable to EL4 cells. Moreover, EL4-rho(0) cells produced ROS at levels similar to EL4 under these conditions. GzmB-mediated ROS production was almost totally abolished in both cell lines by the pan-caspase inhibitor, Z-VAD-fmk. However, addition of apocynin, a specific inhibitor of nicotinamide adenine dinucleotide phosphate (NADPH) oxidases, led to a significant reduction of ROS production and cell death only in EL4-rho(0) but not EL4 cells. These data suggest that gzmB-induced cell death is accompanied by a caspase-dependent pathway of extra-mitochondrial ROS production, most probably through activation of NADPH oxidase. |
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| DOI: | doi:10.1038/icb.2010.5 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: https://dx.doi.org/10.1038/icb.2010.5 |
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| | Volltext: https://onlinelibrary.wiley.com/doi/epdf/10.1038/icb.2010.5 |
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| | DOI: https://doi.org/10.1038/icb.2010.5 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Animals |
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| | Apoptosis |
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| | Caspases |
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| | Cell Line |
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| | Enzyme Activation |
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| | Granzymes |
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| | Humans |
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| | Immunoblotting |
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| | Mice |
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| | Mice, Inbred C57BL |
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| | Mitochondria |
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| | NADPH Oxidases |
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| | Reactive Oxygen Species |
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| | Reverse Transcriptase Polymerase Chain Reaction |
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| | Signal Transduction |
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| | T-Lymphocytes, Cytotoxic |
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| K10plus-PPN: | 1757918639 |
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| Verknüpfungen: | → Zeitschrift |
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Granzyme B of cytotoxic T cells induces extramitochondrial reactive oxygen species production via caspase-dependent NADPH oxidase activation / Aguiló, Juan [VerfasserIn]; 02 February 2010 (Online-Ressource)
